Study On The MCF-7 Cell Cycle Arrest Induced By Se-containing Polysacharide SeGLP-2B-1 From Se-enriched Ganodema Lucidum

Se-containing polysaccharide have very significant preventive effect on many diseases. Se-containing polysaccharide is a polysaccharide product. The study on Se-containing polysaccharide is still in early stages in China.. As a semi-synthetic, semi-natural selenium polysaccharide, SeGLP-2B-1 is mainly obtained through the biological transformation. It has a variety of physiological activities, such as affecting the cell cycle, causing antagonistic effect on strong heavy metal through antioxidant function. .It can promote tumor cell apoptosis, inhibit cell growth,and block the DNA synthesis of cancer cells.Through SeGLP-2B-1 acts on cultured MCF-7 breast cancer cells, we studies the affects on MCF-7 cell cycle and the mechanism of action. The results show that after 72 hours that the SeGLP-2B-1 act on breast cancer MCF7 cells, the tumor cells is marked inhibited, the highest inhibition rate reaches to 80.53% and IC50 is 0.158μM / L. After SeGLP-2B-1 act on breast cancer MCF-7 cells, the cell cycle G1 phase cells increases to some extent, S phase cells marked decrease, S phase cells show the situation first increase and then decrease, indicating SeGLP-2B-1 causes cell cycle G1 / S phase, S/G2 phase arrest.For further research.we studied the cell cycle associating proteins, protein kinases and protein kinase inhibitors The results show that the cell cycle regulatory proteins which contains cdk4,cdk6 and cyclinD₁ protein relates to obviously changed Their expression decrease by SeGLP-2B-1 action time extending. But the expression of protein kinase and protein kinase inhibitors, such as p16, p21, p53 proteins increase significantly SeGLP-2B-1 stimulates the changing of cycle regulatory protein and then causing cell cycle can not move forward smoothly, cell cycle is blocked.Meanwhile, the study founds that cell-surface receptors, cell surface receptor Fas protein with SeGLP-2B-1 treatment time significantly increases; cells with Fas-L protein SeGLP-2B-1 treatment time significantly decrease,it can explain SeGLP-2B-1 lead in the process of apoptosis by stimulating cell surface Fas and other tumor necrosis factor receptor family to complete. On the cell surface Fas, Fas-L receptor protein, resulting in apoptosis, such as p53 and p16 channels in the activation of key regulatory proteins, the activation of these regulatory proteins on the cell cycle protein cycylinD, cyclinB₁ and cycle-dependent protein factor kinase cdk4, cdk6 and other stimulus, eventually leading to cell cycle arrest and apoptosis in cancer cells.