The Application Of Discovering Herbal Medicine Target By Seldi Protein Chip Technologly

Proteomics is new, the most important content in the 21st century in overall cytobiology.for the medicine, agricultural and the industry innovation,It will provide the brand-new mentality. Proteomics related topics have became extremely active research direction in the medicine molecular biology domain in recent years.To the human protein group,we mainly study the special organization, the cell and the disease,through compare the protein expression atlas in different condition (for example different growth growth time, for medicine around, physiology and pathological state) the quantitative analysis various protein change, thus we may dynamic monitor complex biological function metabolism, regulation and so on in the different system(organization, cell, body fluid and so on).by study the protein group, analyzesing between the disease and the treatment quota relations, is becoming recent development direction.in the clinical diagnosis and the treatmen.Proteomics,as the high flux parallel screening technology, is extremely similar between the theory system and Chinese herbal medicine overall view,dialectical view, dynamic view,especially the "card".It maybe has the intrinsic inevitably relation with the protein group which expresses under some specific environment. We can take the "certificate" as the protein group functional sum under the disease certain stage or some specific condition. Chinese herbal medicine think that the "card" including cause of disease, sickness nature,place of disease degree of seriousness of the disease and so on, is response of inside and outside the factor combined of the organism and it is different in deferent disease course. Differing with a disease syndrome,we can think that the same disease is deferent in expressing being unlike individual on one's body function geneome, unlike a stage or the fair amount of confidence function proteide group. The certificate waits output material foundation a possibility and then reflects level a top at the protein set.Because the disease displays the symptom and the body draft has its molecular foundation, these phenotypes essences are the function gene group and its in mRNA, the function protein group horizontal difference external performance, therefore "card" the essence may think is the disease individual gene and its an expression result protein group. Through the protein group study technology, after the compound prescription, each kind of combination opens the side function the protein expression difference, then determination different compatibility of medicines correspondence gene target and function protein. This not only is advantageous to expounds the side medicine function material base and the prescription compatibility of medicines inherent laws, moreover also may in turn knows "the card" again in the treatment stratification plane the essence.The SELDI technology after the American Self lucky living system company (Ciphergfen Biosystems, Inc, Fremont, CA, USA) researches and develops and the commercialization forms SELDI-TOF-MS, also often is called as the protein fingerprint technology or the flight mass spectrum technology. SELDI-TOF-MS was one new protein group research technique which recently several years developed, it take the matrix assistance laser desorption ionization flight time mass spectrum (matrix-assisted laser desorption ionization time of flight mass spectrometry, MALDI-TOF-MS) as the foundation and makes the improvement, fused the mass spectrum and the chip two big technologies characteristic and the superiority. The protein chip technology union protein group study used in the tumor research already to discover a series of new tumors specificity biology sign, including the prostate gland cancer, the ovarian cancer, the breast cancer, the gastro-intestinal tract tumor and so on, simultaneously also has made some new progress in the tumor pathogenesis aspect. Another Chinese native medicine enters the human body display function the final link mostly is the medicine member and the protein response. Through seeks not the good related protein to the protein group's research; The research wants the molecular pharmacology, the toxicology mechanism. SELDI-TOF-MS can around the keen examination center for medicinal purposes medicine the organism protein group express change, thus can fast discover or discovers the new medicine target member to the medicine sensitive organism protein. This experiment take the Chinese medicine fish rank grass inoculation fluid as the example, the experimental animal (dog) is the research object result indicated that, The injection fish rank grass's dog (remains takes with the dog which injects NS (remains for medicine 1-9 day-long blood serums) takes for medicine 1-7 day-long blood serums) compares, compares, examines has the obvious curve change the protein or the protein is right. The SELDI protein chip technology only can in the keen examination blood serum the protein change, certainly not know any protein, but if unifies the clinical symptom to unify other protein purification appraisal technology method, SELDI will be not allow to neglect in the Chinese native medicine protein group study research. In the Chinese native medicine clinical with the medicine, pharmacology analysis, new medicine development use and medicine toxicity analysis, medicine curative effect aspect and so on appraisal has provided the powerful technical method.