Catalytic Fluorescence, Scattering Method For The Determination Of Hemoglobin, Copper(Ⅱ) And Hydrogen Peroxide

Catalytic fluorimetry method make it a powerful tool for determination of trace components because of its high sensitivity,accuracy an selectivity, resonance light scattering technique is simple manipulation, high sensitivity and convenient. They have been extensively and successfully utilized in various fields experimental conditions with ordinary fluorescence spectrophotometer. They were mainly used to analyse biological macromolecules,medicament,dyes,metal ions,nanometer particles and surfactants. Indigo carmine is a dye, due to its oxidized easily fade, often used as a kinetic spectrophotometric redox indicator, it has yet to report for fluorescence analysis. And yet to be seen using butyl rhodamine B as association particles Resonance Light Scattering method for the determination of hydrogen peroxide reported. In this paper, a new catalytic fluorimetry method was developed for the determination of hemoglobin and copper(Ⅱ), a new resonance light scattering method for the determination of trace hydrogen peroxide, the main work as follows:1.Catalytic fluorimetry determination of hemoglobinA new spectroflurometric method was developed for the determination of hemoglobin based on its catalytic activity of mimetic Peroxidase on oxidation of Indigo carmine in the presence of hydrogen peroxide. The mechanism of the catalytic reaction was studied briefly. The kinetic parameter of reaction and the optimum condition were discussed and the results indicated that hemoglobin concentration demonstrated good linear relationship in the range of 6.2×10-11～7.75×10-8 mol/L with a detection limit of 1.67×10-11 mol/L. The method has been applied to determine the hemoglobin concentration in human blood samples. the recoveries were in the range from 98.8% to 103.2%.2. Determination of trace copper(Ⅱ) by catalytic fluorimetric spectrophotometry methodIn NH3-NH4Cl buffer medium, the catalytic effect of trace copper on oxidation reaction of Indigo carmine by hydrogen peroxide was obvious, based on oxidation products can produce fluorescent in reaction conditions, a new catalytic spectrofluorimetric method for determination of trace copper(Ⅱ) was established. The linear range of determination for Cu (Ⅱ) was 0.2～100ng/mL.with a detection limit of 0.05 ng/mL. The method could be applied to determine trace copper in rice and flour and tea samples with satisfactory results. The recoveries were in the range from 96.2% to 104.9%.3. Resonance light scattering method for the determination of trace hydrogen peroxideIn Britton-Robiso buffer solutions at pH 1.98, the oxidizing reaction between H2O2 and KI was to generate I2, which combined with excessive I- and formed I3, And the I3 reacted with butyl rhodamine B to produce b-RhB-I3 association particles, causing resonance spertra increase of absorption value at 583 nm. Consequently, a resonance spertra method was proposed to detect H2O2. The linear range of determination for H2O2 was 3.2×10-7～1.0×10-4 mol/L with a detection limit of 5.384×10-8 mol/L. The method could be applied to determine trace H2O2 in rainwater and disinfactant liquors samples with satisfactory results. The recoveries were in the range from 96.20% to 101.3%.