Study On The Microfluidic Fabrication Of Monodispersed Drug-loaded Microspheres And In Vitro Release

Monodispersed drug-loaded microspheres have been the focus in the research of sustained and controlled release pharmaceutical dosage form due to their targeting to specific tissues and organs and excellent sustained and controlled drug releasing. Traditional mechanical mixing has been employed as the main techniques of microsphere preparation at present. However, this method does not give a precise control over the microsphere size, size distribution and microsphere morphology, and microspheres prepared by this method have a low drug-loading efficiency and poor drug sustained releasing performance.Microfluidic technology is an ideal approach to prepare monodispersed drug carriers in recent years. It can perform sample preparation, reaction, separation and detecting on a microfluidic chip with microchannel network and several functional units. In this experiment we prepared monodisperse pirarubicin-loaded polylactic microspheres using hydrophilic modified T-junction microchannel device, characterized the size and morphology of microspheres, and studied the drug release behavior of microspheres. The results showed that, the microspheres prepared by microfluidic technology had a uniform size and sphere and sustained drug releasing.We also prepared 5-Fu loaded chitosan droplets by flow focusing chip, and then cross-linked to monodispersed microspheres by glutaraldehyde and tripolyphosphate respectively. We characterized and contrasted the size and morphology of microspheres, and then compared the drug-loading efficiency and drug releasing behavior of them. The results showed that,5-Fu-CS microspheres prepared by this method had an excellent monodispersity, drug encapsulation efficiency and sustained releasing.