Development And Validation Of An Analytical Method For The Determination Of Aspirin And Salicylic Acid

Aspirin (ASA) is most widely used as an analgesic, anti-inflammatory and antipyretic drug in the world. people have found some new pharmacological effect of aspirin continuously. It has very short plasma half-life (approximately 20 min), rapidly metabolized to Sali-cylic acid (SA) in the body. At present, most of the method determining the ASA and SA in biological matrix could not meet the requirement of analysis, because of the LLOQ, In this experiment, a sensitive analytical method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for determination of ASA and SA in monkey plasma has been developed. The multiple reaction monitoring mode in negative electrospray ionization in multiple was used with ion transitions of m/z 179.1→137.3,136.8→92.8 and 183.1→141.3 for ASA, SA and Acetylsalicylic Acid-d4 (IS). Protein precipitation with acetonitrile was used to extracted ASA, SA and Acetylsalicylic Acid-d4 (IS) from plasma in the presence of 0.5% formic acid. The compounds were separated on a reversed-phase column with an isocratic mobile phase consisting of water% containing 0.05% formic acid and acetonitrile (60:40, v/v). The method had been validated according to the FDA (Food and Drug Administration) Guidance for Industry:Bioanalytical Method Validation successfully. This method has high sensitivity,show good reproducibility and need small sample volume, it can successfully be used in the drug research.