Expression Of Inhibitor Of Differentiation 2 In Congenital Pelviurereric Junction Obstruction

Purpose:To explore the mRNA expression of inhibitor of differentiation 2 (Id2) in ureteropelvic junction tissue with congenital ureteropelvic junction obstruction (UPJO) in children.Material and Methods:Twenty cases with UPJO and 10 cases with Wilms'tumor without invading into ureteropelvic junction tissue as control group were collected. UPJO were categorized into three groups:renal pelvis dilation, narrow ureter and normal ureter below narrow ureter according to their locations. Control was divided into two groups:renal pelvis and ureter. Semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of mRNA in these tissues. All the data were analysed by t-test and one-way anova.Results:UPJO:No significant difference of the Id2 mRNA expression was observed between renal pelvis dilation and narrow ureter in UPJO (P>0.05), and we found that their expressions were lower than the normal ureter below narrow ureter in UPJO(P<0.05). Control group:the renal pelvis and ureter were similar (P>0.05). UPJO and control group:the Id2 mRNA expression of renal pelvis dilation and narrow ureter in UPJO was lower than renal pelvis and ureter in control group (P<0.05).but the Id2 mRNA expression of the normal ureter below narrow ureter in UPJO was similar to that of renal pelvis or ureter in control group (P>0.05).Conclusions:The Id2 gene may play an important role in congenital UPJO.