Expressions Of GRO-1 And Thrombin Gene In Hepatocellular Carcinoma And Their Correlation Research

AIM:The aim of the present study is to investigate the expression of GRO-1 and Thrombin mRNA in HCC and the relationship between these factors and the prognosis of HCC patients.METHODS:Real-time reverse transcriptase-polymerase chain reaction was performed to observe GRO-1 and Thrombin in 55 cases of HCC tissues and 13 cases of optimum hepatolage tissue.RESULTS:The mRNA expression of GRO-1 and Thrombin were found in all HCC tissues.The expression level of GRO-1 and Thrombin gene in HCC tissues were 1.54±0.62(mean±SD),1.18±0.53(mean±SD) There were significant associations between GRO-1 gene expression and some clinical and pathological findings such as metastatic and recurrence(P< 0.05). Significant differences of half survival time occurred among subgroups according to the expression of GRO-1 (P< 0.05). There were significant associations between Thrombin gene expression and some clinical and pathological findings such as recurrence and AFP(P< 0.05). Significant differences of half survival time occurred among subgroups according to the expression of Thrombin (P<0.05). positive correlation was found between GRO-1 and Thrombin (r=0.73,P< 0.01). Multivariate analysis revealed that recurrence was an independent prognostic indicator about survival.CONCLUSION:The expression of GRO-1 and Thrombin in HCC tissues could be regarded as a valuable indicator in estimating the metastatic and recurrence of HCC patients.