The Expression And Significance Of VEGF And Survivin In Anovulatory Dysfunctional Uterine Bleeding

OBJECTIVE:To investigate the expression of vascular endothelial growthfactor(VEGF) and survivin in endometrium of patients with anovulatorydysfunctional uterine bleeding(DUB) and normal endometrium,and to exploreits correlation with clinical behavior and pathological characters ?? as toinvestigate their value as a marker and clinical prognostic indicator.Methods:47 cases of endometria of anovulatory dysfunctional uterinebleeding patients and 23 cases of endometria of eumenorrhea female wereselected randomly to detect the VEGF and survivin of exemplar byimmunohistochemical method.And the correlation between VEGF and survivinwere analyzed ,too.They were obtained from the Department of Gynaecologyand Obstetrics clinic service of the First Affiliated Hospital of Guangxi MedicalUniversity collected between January 1th and December 31th in 2009. Results: 1.The positive rate of VEGF expression in the endometria ofanovulatory dysfunctional uterine bleeding was obviously lower than thepositive rate of normal endometria, with the ratio of VEGF expression were53.19% and 78.26% respectively. The difference was significantly between twogroups(P <0.05.2.In the endometria of anovulatory dysfunctional uterine bleeding, thepositive rate of VEGF expression in proliferative phase, simply hyperplasia andcomplex hyperplasia were 66.67%, 50.00% and 38.46% respectively. There wasno sgnificance difference??P>0.05??.3.The positive rate of VEGF expression in the endometria of simplyhyperplasia was 50.00%, there was no sgnificance difference between this groupand contrast group??P>0.05??.The positive rate of VEGF expression in theendometria of complex hyperplasia was 38.40%. The difference between thisgroup and contrast group was sgnificance(P <0.05??. The positive rate of VEGFexpression in the endometria of proliferative phase was lower than contrastgroup, but there was no sgnificance difference??P>0.05??.4.The positive rate of survivin expression in the endometria of anovulatorydysfunctional uterine bleeding was obviously higher than the positive rate ofnormal endometria, with the ratio of survivin expression were 61.70% and30.43% respectively. The difference was significantly between two groups(P<0.05). 5.In the endometria of anovulatory dysfunctional uterine bleeding, thepositive rate of survivin expression in proliferative phase, simply hyperplasiaand complex hyperplasia were 50.00%, 68.75% and 69.23% respectively. Therewas no sgnificance difference??P>0.05??.6.The positive rate of survivin expression in the endometria of simplyhyperplasia and complex hyperplasia were 68.75% and 69.23% respectively.The differences between these two groups and contrast group were sgnificance(P<0.05 ?? . The positive rate of survivin expression in the endometria ofproliferative phase was higher than contrast group, but there was no sgnificancedifference??P>0.05??.7.In the endometria of anovulatory dysfunctional uterine bleeding, theexpression of VEGF was downregulated correlation with survivin??r=-0.517??P<0.05??.Conclusion: 1.VEGF immunoreactivity was significantly lower in theendometria from patients with anovulatory dysfunctional uterine bleeding. Itsuggests that VEGF lowexpression may disturb angiogenesis and permeability,and trigger irregular dysfunctional uterine bleeding.2.The overexpression of survivin in the endometria of anovulatorydysfunctional uterine bleeding may decrease apoptosis and promote endometrialhyperplasia, which is concerned with irregular dysfunctional uterine bleeding.3.There were negative correlation between the expression of VEGF and survivin in endometrium of patients with anovulatory dysfunctional uterinebleeding. The results suggest that VEGF downregulate survivin expression.