Effects Of Alcohol Exposure During Pregnancy On Retina In Filial Mice

The eyes are the windows of the soul, it can easily be through the eyes of emotional communication, and accepted by the human brain most of the external information passing through the visual system and the processing completed, this series of process occurs first in the retina, retinal As the structure and function of the nervous system is relatively simple and complete anatomy, has been the field of life science research focus. From outside to inside the retina in 10 layers: retinal pigment epithelium, photoreceptor cell layer, external limiting membrane, outer nuclear layer, outer plexiform layer, inner nuclear layer, inner plexiform layer, ganglion cell layer, nerve fiber layer and inner limiting membrane . bipolar cells and horizontal cell bodies are located in the middle of the inner nuclear layer and outer edge of the bipolar cells were mainly coming from the photoreceptor cells after signal processing by chemical synaptic transmission to ganglion cells, and between the level of photoreceptor cells between cells and bipolar cells, it is mainly the feedback received spread photoreceptor photoreceptors and bipolar cells, and cells in these three complex synaptic connections formed between the network layer, the outer plexiform layer. In the interpretation of retinal development, the people of the toxic effects of alcohol and offspring relationship between retinal development, in particular the impact of research on neuronal rarely reported. The toxic effects of alcohol drinkers can cause multi-system itself, multiple organ damage, mainly affecting the brain, liver, cardiovascular system and immune system; and alcohol during embryonic development have been exposed, it will result in the development of the embryo than serious injury, or even lead to fetal alcohol syndrome (Fetal alcohol syndrome, FAS) from happening. In this study, alcohol exposure during pregnancy through the establishment of animal models to simulate the occurrence of fetal alcohol syndrome, immunocytochemistry and DiI scattering techniques, alcohol exposure during pregnancy in different age sub-retinal bipolar cells and horizontal cells for system development study, the results are as follows:1. Alcohol exposure during pregnancy affect the development of child's retinal bipolar cells Using immunocytochemical methods, models in the control group and alcohol group on P7, P14 and P30 mice of all ages scripts development of retinal bipolar cell morphology in general, the results showed alcohol compared with the control group model bipolar cells In its development process as a general morphological differences, such as reducing the number of cell bodies, axons by reducing the density decreases and so, in the high dose group, these differences of alcohol more pronounced, indicating that alcohol exposure during pregnancy affect the sub-retinal bipolar cell development, and a dose-dependent effects and long-range effect.2. Alcohol exposure during pregnancy affect the development of sub-retinal horizontal cellUsing DiI scattering, in the control group and model group on P7, P14 and P30 each age level piece retinal cells were marked and observed the level of the general morphology of cell development, the results showed alcohol levels of exposed group than in the control group, cells in the developing there are obvious differences, such as reducing the number of dendrites, dendritic length longer short, horizontal cell receptive field decreases and so, especially in the high-dose alcohol group, these changes more evident, indicating that alcohol exposure during pregnancy affect the child retinal horizontal cells development, and a dose-dependent effects and long-range effect.In short, the retina and visual form in the visual transduction plays a very important role, through the study of this issue, so we further recognize that alcohol abuse during pregnancy on the offspring of the harm caused by alcohol exposure during pregnancy in particular, will affect the offspring of retinal development, and its influence on the offspring of the retina in a dose and long-range effect, which is explained as caused by exposure to alcohol during pregnancy-related eye diseases such as amblyopia, strabismus and other pathogenesis are of great significance, and this For prenatal and postnatal care, and enhance people's health has tremendous social benefits, on neurotoxicology, learning and developmental neurobiology eye also has got to theoretical value.