| The preparation of Complete Biological Small-Caliber Blood Vessel GraftObjective With the growing disease rate of the coronary atherosclerotic herat disease (CAHD), the coronary artery bypass grafting (CABG) was great used in the clinic. However, the autogeneic blood vessel graft were finite and tissue engineering blood vascular (TEB) cost too much time for made. Our experiment made a new complete biological small-caliber blood vessel graft, offer a new graft for the CABG.Methods Canine bilateral carotid arteries (n=20) were decellularized by enzymatic and detergent extraction procedures. Then the arteries were randomly divided into two groups:group A (decellularized and heparin treated) and group B (only decellularized). Observed the removing situation of the decellularized arteries with HE stain, examed the efficiency of heparin combine with toluidine blue staining. The diameter of the arteries of the two groups were measured and compared with human's saphenous vein during operation.Results After the enzymatic and detergent treatments, the result of the HE stain shown the cells of the grafts were removed completely and the matrix are protected well. The toluidine blue staining shown the heparin was combined with the whole vascular wall of group A, the group B gave a negative result. There was no statistical significance in diameter of the grafts in group A, group B and human's saphenous vein (P>0.05).Conclusions After the enzymatic and detergent treatments, the the cells of canine carotid arteries were removed completely and the matrix are protected well. After the decellularized and heparin treated,heparin was combined with the whole vascular wall. The treated canine carotid arteries could simulate the human's saphenous vein. Objective Establishing a bilateral commom carotid artery bypass graft model on the rabbits, to simulate the coronary artery bypass grafting.Canine carotid arteries were decellularized and coated with heparin to develop a small-caliber vascular graft. Then evaluate the feasibility of the new graft at this model.Methods Self-controlled study is carried on the 20 rabbits to establishing a bilateral carotid artery bypass graft model. The grafts of group A were transplanted into the left carotid arteries, while those of group B were transplanted into the right side. The diameter of the bilateral carotid arteries was measured and compared with the coronary arteries of 20 patients with coronary heart disease (CHD). The patency of the grafts of the two groups was observed with B-type ultrasonography after 3 weeks and 3 months. Sacrificed the rabbits after 3 months and observed the remolding situation of the graft artery by HE stain and electron microscope,examed the heparin combine information of the group A which were transplanted for 3 months by toluidine blue staining.Results The diameter of the carotid arteries of the rabbits and the coronary arteries of the patients was no statistical significance.Thrombus was found in some grafts in both sides. Overall, the patency rate was found significantly better in group A than in group B. The patency rate was 95.0% (group A) versus 40.0% (group B)(P<0.05) at 3 weeks, and the thrombosis rate was10.0% (group A) versus 70.0% (group B)(P<0.05) at 3 months. The HE stain and electron microscope found that the grafts without thrombus formation showed a satisfactory cellular reconstruction. The toluidine blue staining to group A still shown weakly positive after 3 mouths.Conclusions The bilateral carotid artery bypass model on the rabbits is easy to establish and can be used to explore and evaluate the small-caliber bypass vascular grafts for coronary bypass grafting. Canine common carotid artery treated by detergent and enzymatic extraction and heparin treatment released slowly during the 3 months and may become a new complete biological small-caliber vascular xenograft.
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