The Effects Of Glargine, Detemir And Human Insulin On The Proliferation And Differentiation Of 3T3-L1 Preadipocytes

Objective: To observe the effects of glargine, detemir and human insulin on the proliferation and differentiation of 3T3-L1 preadipocytes.Methods: 1. 3T3-L1 preadipocytes were cultured in vitro, its proliferation were detected by MTT method every second day after adding glargine (including powder and solution), detemir (solution) and human insulin (powder) with the concentration of 10 nmol/L in the culture until the twelfth day. 2. 3T3-L1 preadipocytes were cultured in vitro, its differentiation were detected by oil red O staining method every second day after after adding glargine (including powder and solution) , detemir (solution) and human insulin (powder) with the concentration of 0.25μmol/L in the culture until the fourteenth day. 3. Statistics analysis: Values presented were mean values±S.E. Many groups of quantitative data were compared by one-way ANOVA, multiple comparison used LSD-t test. The threshold for statistically significant difference was P≤0.05.Results: 1. MTT staining showed an absorbance of glargine group (powder 0.697±0.068) and human insulin group (powder 0.613±0.077) significantly higher than that of control group (0.549±0.072) (P<0.05) from the sixth day, and the absorbance of glargine group (powder) was significantly higher than that of human insulin group (powder); MTT staining showed an absorbance of glargine group (solution 0.693±0.053) and detemir group (solution 0.657±0.042) significantly higher than that of control group (0.549±0.072) (P<0.05) from the sixth day, and the absorbance of glargine group (solution) was higher than that of detemir insulin group (solution). 2. Insulin was an essential ingredient during the differentiation period of preadipocytes; the absorbance of glargine group (powder) was lower than that of human insulin group (powder) in oil red O staining (P<0.05), and the difference was the most significantly at the twelfth day (0.691±0.029 vs. 0.769±0.045); the absorbance of detemir group (solution) was lower than that of glargine group (solution) in oil red O staining (P<0.05), and the difference was the most significantly at the twelfth day (0.447±0.041 vs. 0.531±0.036).Conclusion: Insulin could promote the proliferation and differentiation of 3T3-L1 preadipocytes, but there were difference among the effects of glargine, detemir and human insulin.