Induce Of Immature Dendritic Cells In Micro-environment By Pancreatic Cancer Cells

Obiective:To study the effect of pancreatic cancer microenvironment on maturation of dendritic cellsand to investigate the mechanism which tumors escape from immune recognition.Method:DC were cultured with rmGM 40ng/ml, rmIL-4 40ng/ml and supernatant of cell line AR42Jwas added on day 6, then a large number of DC were gained. DC cultured and induced withregular methods were used as control. The expression of surface molecules CD86, CD80 of DCwas detected to observe whether this intervention can delay or block maturity of DC and thiseffect can be reversed by be stimulated with lipopolysaccharide (lipopolysaccharide, LPS). AndT cell stimulatory effects were determined.Result:Compared to DC surface molecules CD86,CD80 expression significantly decreased in Dcinduced by Pancreatic cell cultured supernatant,CD80,CD86 molecules from 71.5 % to 8.0%,theCD86, CD80 expression is still low of Dc stimulated by LPS,it shows that there is a block effctabout pancreatic cancer cell culture supernatant on Dc.And Allostimulatory capacity of AR42Jcell culture supernatants to stimulate the imDCs group was much lower than that of the normalcultivation of imDCs group.Conclusion:The inhibitory effects of supernatant from Pancreatic cancer cells on the maturation ofDCs,And this immature state is not easy to reverse.And Allostimulatory capacity of AR42J cellculture supernatants to stimulate the imDCs group was much lower than that of the normalcultivation of imDCs group.