The Effect Of Ischemic Postconditiong And Glutathione On Ischem Reperfusion Injury Of Limbs In Rats

Objective:There are many situation Which libms are subjected to ischemie-reperfusion (IR) injury. This pathophysiology course lies in acteriosclerosis obliterans bypass graft,thrombosis of major blood vessels,transplatation and application of tourniquet. It doesn't only cause limb nonfunction but also can lead to multi-organ injury ,such as the nonfunction of the liver, kindney, lung and so an. How to lessen the damage of ischemical-reperfusion injury is a warm spot that be studied. Until resently,there are many mechanismsof the ischemical-reperfusion injury: ealcium overload,effect of the oxygen free radical and NO, the participate of various kinds of cytokines and the stimulating of endotheliocyte making the microcireulation disturbanee,and so on. In this study we estsblished the model of libms ischemie-reperfusion injury, evalute the activity of SOD and the level of MDA in the blood serum. By observing impact of the ischemic postconditioning and reduced glutathione on the above index,to probe the mechanism of their Protection on the ischemical-reperfusion injury, to provide a dependable experiment more basis For the clinical application.Methods:Forty Wistar rats were randomly allocated into 5 groups(n=8): groupⅠsham operation(S); groupⅡischemia-reperfusion(I/R); groupⅢI/R+ischemic postconditioning(IPo); groupⅣI/R + reduced glutathione(GSH); groupⅤI/R+ischemic postconditioning + reduced glutathione(IPo+GSH).we estsblished the model of libms ischemie-reperfusion injury. The abdominal aorta was exposed through midline incision and occluded 2 h with mini-clamp then unclamped for 2 h. In S group theabdominal aorta was exposed but not clamped. In IPo group, the abdominal aorta was induced by 3 circles of ischemia(5 min) separated by reperfusion(5 min) before reperfusion for 2 h. In GSH group, reduced glutathione 120 mg/kg was given i.v. before reperfusion for 2 h. In IPo +GSH group, the abdominal aorta was induced by 3 circles of ischemia(5 min) separated by reperfusion(5 min) after ischemia for 2h and then reduced glutathione 120 mg/kg was given i.v. before reperfusion for 2 h. After reperfusion for 2 h, get blood from theinferior vena cava,and measure destety of MDA and the aetivity of SOD. The results were measured by mean±SD,analysis of variance was used to test for differences on SPSS13.0,least significant difference test (LSD-t) for multiple comparison.Results: After I/R,the MDA contents was significantly increased while the SOD activity was significantly decreased as compared to the S group(P<0.05). The MDA contents was significantly lower while the SOD activity was significantly higher in IPo, GSH, and IPo+GSH groups as compared with I/R group(P<0.05). The damage induced by I/R were significantly ameliorated.The MDA contents was significantly lower while the SOD activity was significantly higher in IPo+GSH group compared with IPo and GSH groups(P<0.05). There were no significant difference in the indexes among the IPo group and GSH group(P>0.05).Conclusion: IPo, GSH and Ipo+GSH can reduce the risk of limb dysfunction and injury associated with renal I/R injury in rats through enhancement SOD activity. IPo, GSH and IPo+GSH can protect the limbs against ischemia-reperfusion injury in rats and IPo+GSH is more effective.