Inhibition Effect Of Manumycin On Cell Growth Of Human Myeloblast Leukemia Cell K562 And Its Mechanism

Objective:Farnesyl transferase inhibitors are a new class of signal transduction inhibitors,The vitro experiments have confirmed that theFTI can inhibit the growth of breast cancer cell line MCF-7,Thyroid cancer cell line,Hepatoma cell line,Pancreatic cancer cell line and lung cancer cell line.Manumycin was derived from the cultivation liquid of Streptomyces,whose amide side chain is similar to the isoprene groups of Farnesylpyrophosphate, Manumycin is Low toxicity and high specificity, but its mechanism is not very clear.Based on the above,our experiments are intended to use Manumycin in human leukemia cell line, In order to observe the effect,and further study related mechanisms.Broaden the application range of Manumycin,for the treatment of leukemia search for new potential.Method:We used 2,4,8,16 as the end concentration of Manumycin,the K562 cell were cultured with different concentration of Manumycin about 24h,48h and 72h, Methyl thiozolyl tetrazolium (MTT), was used to observe the growth suppressive rate of these cell.then we put K562 cell in different concentration of Manumycin about 24 h,western blot was used to detection the expression of survivin protein.Results:The growth condition of drug groups are worse than the control group,The Manumycin can inhibit the proliferation of K562 cell with time and dose-response,Manumycin could down-regulate the expression of survivin.The experiment provided theoretical support and experimental basis for the new treatment of leukemia.Conclusions: 1.We observed that the Manumycin could inhibit the growth of K562,which were incubated with various concentrations of Manumycin The inhibition effect was time and dose-response relationship.2.Manumycin could down-regulate the expression of survivin protein,with the increase of Manumycin concentrations, the expression of survivin protein gradually reduced.3.Manumycin could inhibit the proliferation of K562cell by down-regulating the expression of survivin protein to achieve the purpose of treatment of leukemia.