Study On The Plasma Level Of Urotensin Ⅱ In Artherosclerosis Rabbits Model

IntroductionUrotensinⅡ(UTⅡ)was konwn to be the most vasoconstriction peptide,which widely distributed in the tissue of heart,kidney,vessel soomth muscle,even artherosclerosis plaque.It palys a key role in the mechanism of artherosclerosis plaque formation.In our study,the model of artherosclerosis model was made with rabbits,we investigate plasma change of urotensinⅡ,the relationgship between IL-6,TC.Make clear the important effect of urotensinⅡin the mechanism of artherosclerosis.PurposeThis study designed to investigate the difference of the plasma level of urotensinⅡthe relationship between urotensinⅡ,IL-6and TC.Material and methods1.material40 healty rabbits weighing 2.0-2.5kg,were obtained from department of animals,shenyang pharmaceutical university.2.preparation of high-fat diet The basic stoyer consist 0.6%cholest,3.3% lard,8.3% egg yolk to make the high-fat diet.3.Animal treatmentRabbits were randomly divided into two groups,the control group and the artherosclerosis group,each group contain 20 rabbits. The control group keep feding with basic rabbit stoyer, artherosclerosis group,fedding with high-fat diet.every day,each artherosclerosis group rabbit eats 150g stoyer with contain choles t1.0g, lard 5.0g, egg yolk 12.5g.the study continue 12 weeks.4.detection of the biochemical projectsWe detected the rsbbits serum total choletrol,triglyceride,low density lipoprotein-cholesterol, high density lipoprotein-cholesterol. urotensinll and IL-6 were determined by elisa method.5.statistical analysisThe result were processed by SPSS13.0 software.differences between the group were analyzed by Analysis of Variance(ANOVA).Results1.Difference of body weight and serom lipid of rabbits in each groupThe difference of weight between rach has no significant sense. (P>0.05).serum lipid especially TC and LDLC had predominance higher when compare to control group and artherosclerosis grou.12weeks experimental group make obvious higher than 6weeks experimental group. (P<0.01)。2.Difference of plasma UTⅡand IL-6 in each groupThe plasma level of UTⅡin high-fat diet group goes down at 3weeks.12weeks level make big difference between control group and 6weeks group, (P< 0.01).Compared 6weeks and control group level of IL-6,the 12week high-fat diet group increased significant, (P<0.01)3.the relationship between UTⅡ,L-6 and TCIn our study we can see negative relation between UTⅡand TC, regression equation Y=0.211-0.003X r=-0.99, P=0.01. there was also negative relation between UTⅡand IL-6 regression equation Y=0.513-0.002X,r=-0.95, P=0.04。 conclusionUTⅡparticipate the foremation of artherosclerosis,goes decrese of plasma level. UTⅡmake obviouse negative relation between serum lipid and IL-6.There was significant upgrade of serum lipid level, especially TC.The inflammation reaction penetrate the formation of artherosclerosis,they interact tightly,make key role in artherosclerosis formation.