Organization Inductive Nerve Conduits Framework And Function Of Evaluation

Objective:The objective was to fabricate a novel tissue inducible nerve conduits, and Functional evaluationMethods:The microspheres were prepared with chitosan that coated with ligustrazine, application of the controlled release method to detect the release effect of the chitosan microspheres/ligustrazine in vitro chitosan microspheres coating ligustrazine were mixed with collagen to fabricate the tissue inducible nerve conduits, The nerves conduit were cross linking with genipin after freezed dehydration using the gradient cryotechnique. The scanning electron microscope (SEM), was used to observe respectively the spatial structure of the never conduit, the distribution of the microspheres.the mechanical characteristics of the conduit were evaluate by the almighty materials testing machine. Mesenchymal Stem Cells (MSCs) were separate from the bone marrow and identification. MSCs were induced with ligustrazine for different times, the effect of MSCs differentiation into never cells with ligustrazine were analyzed by RT-PCR and Wester blot methods. MSCs were co-cultured with never conduit, The methods, such as light microscope and Immunocytochemistry, were used to study the effect of the tissue inducible nerve conduits on MSCs. MSCs combined with extracellular matrix (ECM) that inoculated in the entocoele of the conduit were culture for 7 d and 14 d, the tissue compatibility were observed by SEM.Results The controlled release in vitro showed that the chitosan microspheres had good release effect. The structures of the never conduits were significantly alterated in cross linking by genipin. collagen were arranged compactly after cross linking, which had a certain quantitative pore, the microspheres were uniform distribution on the surface. the largest load and breaking load of the collagen conduit after crosslinking were significant increase than that of befor crosslinking. ligustrazine could induced MSCs expression Nestin, NSE, P-Tubulin III, Nurrl mRNA and NSE protein. The nerves conduit had the favourable histocompatibility with MSCs. The never duct that contained ligustrazine could promote MSCs differentiation into never cells and had the function of tissue inducible.Conclusion:1. The chitosan/ligustrazine microspheres had good release effect.2. The fabricated nerves conduit had the favourable mechanical characteristics and histocompatibility.3. The ligustrazine could induce MSCs differitiation into never cells,4. The ligustrazine were controlled release from nerves conduit, which could induce MSCs differitiation into never cells, the never conduit had the function of tissue inducible.