Research On The Relevance Of The Different Phases In RAT Sleep And The 5-HIAA Contents In Nucleus Raphe Pontis And The Mechanism Of SNS In Sleeping Improving

Objective: To investigate the correlation between sleep cycle and 5-HT content and neurotransmitter mechanisms of SNS to improve sleep. Method: In this experiment, rats were observed by using the nervous system biochemical analysis, application of multi-operation polysomnography (PSG) and deprived rats sleep by electrical stimulation to determine the impact of sleep phase on freeze-dried powder of SNS. On this basis, using brain microdialysis combined with EEG, EMG judgments, clear and accurate collection rat cerebrospinal fluid in raphe nucleus during the period of wake and sleep.Using high performance liquid with Electrochemical detection, measured levels of 5-HT or its metabolite 5-HIAA in rats raphe nuclei during awake and sleep. Use the contents as a target to discuss the relativity between sleep cycles and 5-HT content and mechanisms to improve sleep of SNS.Result:1. SNS freeze-dried powder (87.5mg/ml) ig for 9 days, significantly reduced wake time,(Wake) (P <0.01), significant extended total sleep time (TST) (P < 0.01), mainly to extend the slow wave 2 (SWS2) (P <0.01) and REMS (P <0.05), can extended SWS2 near normal, and no significant effect on slow wave sleep 1 (SWS1).2. Compared with normal brain of 5-HIAA levels on awake phase in raphe nucleus, SWS1 and SWS2 of 5-HIAA levels were lower, both were significant and extremely significant difference (* P <0.05, ** P <0.01).3. Compared with model group (electrical stimulation deprive sleep) of 5-HIAA levels on awake phase in raphe nucleus, SWS1 and SWS2 of 5-HIAA levels were lower, both were significant and extremely significant difference (* P <0.05, ** P <0.01).4. Compared with the control group, the content of 5-HIAA increase, they has significantly different (p <0.05 *). After giving SNS, SWS1 and SWS2 period of 5-HIAA levels were lower, SWS1 period compared with the model group were significantly different (p <0.05 #) SWS2 of no significant difference (p> 0.05).Conclusion:1.SNS lyophilized powder (87.5mg/ml) ig for 9 days, with improved sleep function, its role is characterized by: the awakening of rats to reduce sleep deprivation time (Wake), extended SWS2 (P <0.01) and REMS (P <0.05), to a total sleep time (TST) to extend2. Compared with the awake state, induced by electrical stimulation in normal rats or rats of sleep deprivation in sleep, SWS1 and SWS2 raphe nucleus of the brain during the 5-HIAA levels were reduced.3.SNS freeze-dried powder to improve the sleep mechanism is related to the 5-HT content of brain raphe nucleus.