Preliminary Study On Genotype Of Hepatitis B Virus Detected In Xinjiang

Objective:Genotype is a useful tool in understanding the epidemiology and immunology of HBV infection now. Nucleotide variability between HBV genotypes has been used to trace routes of HBV transmission. And there is increasing evidence that the clinical picture, the response to treatment and the long-term prognosis may differ depending on which genotype has infected the patient. In my study, I used the method of sequencing and phylogenetic analysis to determine the distribution of genotypes, subtypes and S mutation of HBV detected from Xinjiang.Methods: Serum samples were collected from HBsAg carriers of Xinjiang People's Hospital. The S gene was amplified by nested PCR and the PCR products obtained were used for direct sequencing. The viral genotype was identified after phylogenetic analysis of the sequences obtained and GeneBank reference sequences corresponding to HBV genotype A to H. I confirm that the dominant genotype of HBV detected from Xinjiang and according to the amino acid sequences deduced from the nucleotide sequences of S gene, and then confirm the dominant subtype and S mutation of HBV detected from Xinjiang.Results:(1) The dominant genotype of HBV detected from Xinjiang is genotype C and genotype D, and the minority is B. (2) The majority serotype of HBV detected from Xinjiang is ayw and the minority is adr. (3) The frequency of nucleotide substitution of Community -based HBV S gene was 2.17/100nt. Only 2 nucleotide substitutions bring up sense amino mutants. They are Q129H and M133L, both of them occurred on the first loop. (4) Genotype C was leading in all different clinical patients. Most patients with convalescence,LC and HCC had genotype C and genotype D. Some of ASC and HC had genotype B,C and D. And the ratio of genotype B in HC was increased.Conclusion:In this study,I determine the main genotype and subtype of hepatitis B virus (HBV) prevailed in Xinjiang and the mutation in S gene and the relationship between genotype and the phenotype. It can be used to make a prevention strategy and instruct clinical treatment for the people of Xinjiang.