The Research On The Bioactive Secondary Metabolites Of Two Strains Of Actinomyces Belonging To The Genus Of Streptomyces

Aim To study the bioactive secondary metabolites of two strains of Actinomycetes belonging to the genus of Streptomyces by bioassay-guided fractionation, one strain from the deep sea of Sourth China Sea numbered as 220225 with inhibitory effect against PTP1B, and the other from the root system soil of Cephalotaxus hainanensis from Danzhou Botanic Garden in Hainan Province numbered as 30701 with inhibitory effect against HCT 116. Methods Various chromatographic and spectroscopic methods were applied for the isolation and structure identification of the secondary metabolites, and the bioactivities of pure compounds were also evaluated. Results and Discussion Eleven compounds were obtained from the strain of Streptomyces sp (No.220225), one compound was isolated from nature for the first time, and four compounds were isolated from the genus of Streptomyces for the first time. Pharmacological experiments revealed that eight compounds showed moderate inhibitory effects against PTP 1B. Nine cyclic dipeptides were obtained from the strain of Streptomyces sp (No.30701), and one was isolated from the genus of Streptomyces for the first time. Pharmacological experiments revealed that one compound exhibited weak inhibitory effects against HCT 116 with inhibition rate of 40.6% at a concentration of 20μg/mL. Key words:Streptomyces sp.; Actinomycetes; marine drugs; secondary metabolites; proteintyrosine phosphates 1B (PTP 1B); human colon tumor 116 (HCT 116)...