The Advantages And Clinical Application In Pregnant Women To Be Infected With Toxoplasma By Real-time Fluorescence Quantitative PCR Assay

Objective:Toxoplasmosis is a kind of the epidemic zoonosis in worldwide, both humans and animals can be infected by this kind of zoonosis. Toxoplasmosis is caused by Toxoplasma gondii. Toxoplasma gondii is a specific parasite that parasitic in cells, it can cause the systemic diseases. This kind of parasit can parasitic in the nucleated cells from more than one hundred kinds of vertebrate which included humans, bringing diseases and deaths. In human, its detriment conclude the congenital danger in foetus and the acquired danger after the borning in pregnant women which be infected with toxoplasma, it may cause the foetus'death,deformity and miscarriage. Therefore, it is very important to find out a high-speed and accurate method, to offer the value for Toxoplasmosis detection.Methods:We will use both the ELISA assay and the real-time fluorescence quantitative PCR assay, to detect all the samples that send to the lab first time from the pregnant women who want to have the toxoplasmosis detections in one and half year. These samples are from the four large-scale hospitals in Ganzhou area(The First Affiliated Hospital of Gannan Medical College,The First People's Hospital of Ganzhou City,The City Hospital of Ganzhou,The Women and Children Health Care Hospital of Ganzhou City). Through the comparative reseach, we will find out the superiority and the application in clinical by the real-time fluorescence quantitative PCR assay in the pregnant women which be infected with toxoplasma.Results:There are all 800 samples. ELISA:13 samples TOX IgM-Ab are positive but IgG-Ab are negative; 5 samples IgM-Ab are nagative but IgG-Ab are positive; 30 samples both IgM-Ab and IgG-Ab are positive; 752 samples both IgM-Ab and IgG-Ab are negative; the positive rate is 5.38%(43/800). The real-time fluorescence quantitative PCR assay:45 samples are positive,755 samples are negative, positive rate is 5.63%(45/800). The positive rate of these two assays P >0.05, have no statistically significant. As the real-time fluorescence quantitative PCR assay to be the standard, the sensitivity of ELISA is 95.56%(43/45)Conclusion:The real-time fluorescence quantitative PCR assay is better than ELISA, no matter in its specificity or its sensitivity, and also the testing is more simple and easy to use; but we should do the test in standard, to avoid all the factors that may cause the false positive results during the whole proceed.