Study On Lipoprotein Apheresis With Degraded Polyanionic Polysaccharides

In this paper, the acid mucopolysaccharides such as degraded carrageenan, pectic acid and alginic acid were obtained by degradation of carrageenan, sodiumpectate and pectic acid with hydrogen peroxide. Their properties for removal of total cholesterol (TC), low-density lipoprotein cholesterol(LDL-C), high-density lipoprotein cholesterol(HDL-C) and total protein(TP) from plasma were investigated. The main conclusions were made as follows:1) The degraded Carrageenan was synthesized by degradation with hydrogen peroxide, and the proper process conditions were determined. The molecular weight of the degraded carrageenan for lipoprotein apleresis is about ten thousand. The results of LDL-C purification showed that, at pH value was 5.10 and the concentration of purifying agent was 125mg/dL, the reduction percentage of TC, HDL-C, and LDL-C+VLDL-C,was 66.09%,24.28%,83.67%, 13.45% respectively.2) The degraded alginic acid was synthesized by hydrogen peroxide. The results of lipoprotein removal showed that, under proper conditions, the removal percentage of TC, LDL-C+VLDL-C, HDL-C, TP with degraded alginic acid was 46.3%,56.8%,21.9% and 11.5% respectively.3) Sodiumpectates which was degraded by hydrogen peroxide and hydrogen chloride were prepared. The optimal process conditions for degraded sodiumpectates were obtained. The degraded sodiumpectate was first used as LDL-C purifying agent. The results of lipoprotein apheresis showed that, at pH value was 5.10 and the concentration of degraded sodiumpectate was 140mg/dL, the reduction percentage of TC, HDL-C, and LDL-C+VLDL-C, was 63.1%,24.3%,78.1% and 12.4% respectively. It was proved that the degraded sodiumpectate was a novel agent for LDL apheresis.The purifying agents that synthesized in this paper possess good selective removal of lipoprotein. The paper provides basis for the further development of lipoprotein purifying agents and their applications.