Effects Of Con-G And Its Analogues Inhibiting Morphine Induced Rewarding And CaMKⅡα Expression In Mice

1 ObjectiveIn the present study, we further focus on the anti-morphine psychic dependence effects of Con-G, Glu-Con-G and Glu-Con-G[1-13] by CPP. We used the western blotting to testify the changes of the levels of CaMKⅡαin morphine-addicted mice PFC and Hp to discuss the role of CaMKIIa in morphine psychological dependence.2 Experimental methodsMale Kunming mice (18-20g) were used in this experiment. The experiment was made up of two parts:behavioral experiment(CPP) and the molecular experiment(Western blotting).3 ResultsThe results of behavioral experiment(CPP) shows that, Con-G, Glu-Con-G and Glu-Con-G[1-13] (120,240,480pmol) by single intracerebroventricular administration attenuated the expression and reinstatement phases of morphine-induced CPP, and had no invisible behavioral disorder under our experiment conditions.The results of molecular experiments (Western blotting) showed that, a certain dose of Con-G, Glu-Con-G and Glu-Con-G [1-13] by single intracerebroventricular administration can attenuate the levels of CaMKⅡαin PFC and Hp brain areas of morphine addictive mice.4 Conclusions1. Con-G, Glu-Con-G and Glu-Con-G[1-13] (120,240,480pmol) by single intracerebroventricular administration attenuated the expression and reinstatement phases of morphine-induced CPP.2. Con-G, Glu-Con-G and Glu-Con-G[1-13] (120,240,480pmol) by single intracerebroventricular administration had no invisible behavioral disorder under our experiment conditions.3. The levels of CaMKⅡαwere increased in PFC and Hp brain areas of morphine addictive mice.4. The certain doses of Con-G, Glu-Con-G and Glu-Con-G[1-13] by single intracerebroventricular administration can attenuate the levels of CaMKⅡαin PFC and Hp brain areas of morphine addictive mice.