The Research On Potency Of TMP And Its Mechanism Of Anti-disuse Muscle Atrophy

Objectives:1.To compare the effects of high dose TMP and high dose Ligusticum chuanxiong,which are capable of restressing the unloaded muscle atrophy in a hind limb unloaded rat model, and find out the more effective.2.To study and research the effects of different concentrations of TMP on the soleus's in a hindlimb unloaded rat model,and find out the optimal dose of TMP on restressing the unloaded muscle atrophy.3.To study and research the effects of optimal dose TMP on the function of soleus's contractility and intra-cellular Ca2+-ATPase activity,based on the model of tail-suspended rat,be aimed to explore the effect of TMP for inhibiting the unloaded muscle atrophy.Methods:1,To creat the model of unloaded muscle atrophy rat through suspension the tail of rat for 14 days, and observe the effect of different concentrations of high dose TMP on skeletal Muscle of modeled-rat.2, To survey and evaluate the effects of optimal dose of TMP on rat skeletal Muscle's contractility by the equipment of PowerLab system and electronic stimulator (SEN.3301)3,To testify the of the effects of optimal dose of TMP on skeletal Muscle's intra-cellular Ca2+-ATPase activity,using the method of inorganic phosphate determination.Results:Comparision with HLU+W:1.High dose of TMP and Ligusticum chuanxiong inincrease the CSA of SOL muscle fiber for 33.05%(P<0.001),39.15%(P＜0.001); reduce exchange from type I to type II of muscle fiber for 14.98%(P＜0.001),18.79%(P＜0.001).2.1) the different concentrations group of TMP, HLU+Tmpl, HLU+Tmp2, HLU+Tmp3, HLU+Tmp4, separately raise the CSA of typeⅠfor 39.14%(P＜0.01),18.73%(P>0.05),11.72%(P>0.05) and 8.90%(P>0.05).2.2) increase the CSA of typeⅡseparately for 36.66%(P＜0.001),16.05%(P＜0.05 ),15.93%(P>0.05)和-9.15%(P>0.05). The different concentrations group of TMP, HLU+Tmp1,HLU+Tmp2,HLU+Tmp3,HLU+Tmp4,separately inhibit the exchange from typeⅠto typeⅡof muscle fiber for 18.79%(P＜0.01),14.33%(P＜0.05),13.66% (P＜0.05),1.62%(P<0.05).3.1) the value of soleus muscle Pt affected by the opimal dose TMP raise by 37.22%(P<0.001),Po value relatively raise for 13.03%(P>0.05), TP50, TPT, RT50, RT75 represent obvious discrepancy,and separately increase for 49.65%,37.55%, 16.67%,9.84%, and the discrepancy is notable (P＜0.001).3.2) The value of extensor digitorum longus(EDL) muscle Pt affected by the opimal dose TMP(the goup of HLU+TmpH) relatively raise by 39.58%(P<0.001), Po value relatively raise for 37.47%(P<0.001); TP50,TPT,RT50,RT75 of HLU+TmpH separately increase for 48.24%,34.10%,9.98%, 6.25%, the discrepancy is notable (P<0.01).4.1)The activity of Ca2+-ATPase of soleus muscle in group of HLU+TmpH up-regulate for 25.09%(P<0.05);Compare with CON, the change is distinguished, The activity of Ca2+-ATPase of HLU+W up-regulate for 25.09%(P<0.05), The activity of Ca2+-ATPase of HLU-TmpH up-regulate for 47.10%(P＜0.001).4.2) The activity of Ca2+-ATPase of EDL in group of HLU+TmpH down-regulate 65.28%(P＜0.01).Conclusions:1.TMP is more effective than Ligusticum chuanxiong on restressing the unloaded muscle atrophy,for that TMP tremendously prevent the development of unloaded muscle atrophy.2.To study and research the relationship of concentration and two general index:CSA an on inhibiting atrophy of skeletal muscle, to find out 60mg 10mlkg-1 is the optimal dose of TMP:3.The optimal dose of TMP can up-regulate skeletal Muscle's contractility in a hindlimb unloaded rat model.4. The optimal dose of TMP can up-regulate the activity of Ca2+-ATPase to some degrees in a hindlimb unloaded rat model.5. TMP inhibit the muscle atrophy through below mechanism:up-regulate activity of Ca2+-ATPase, prevent the exchange of muscle fiber percentage,enhance the constriction of skeletal muscle, et al.