Experimental Research Of Salidroside On Intracellular Free Ca²⁺ Concentration Released From Sarcoplasmic Reticulum In Cultured Rat Cardiomyocytes

Objective To study the effects of salidroside on intracellular free Ca²⁺ concentration released from sarcoplasmic reticulum in cultured rat cardiomyocytes and its mechanism.Methods The fluorescent intrasity of intercellular calcium concentration was measured with laser scanning confocal microscope in cultured cardiomyocytes of newborn rats loaded with Fluo-3/AM(5μmol/L).Primarily cultured cardiomyocytes of neonatal rats were divided into 3 groups: control group, different concentrations of salidroside groups, verapamil pretreatment+different concentrations of Sal groups.Results (1) In D-Hanks solution, salidroside at the concentration of 15ug/mL,30ug/mL 60ug/mL can elevate [Ca²⁺]i , salidroside elevated the fluorescent intrasity of [Ca²⁺]i in cardiomyocytes from 524.11±3.11 ,530.61±4.40 , 532.24±3.40 to 574.08±4.65 , 591.86±3.64 , 618.66±4.27 respectively,(P<0.01),which indicate that the level of [Ca²⁺]i act in an dose-dependent.(2)In D-Hanks solution with verapamil, salidroside elevated the fluorescent intrasity of [Ca²⁺]i in cardiomyocytes from 357.74±3.13 ,387.17±2.37 ,391.43±1.34 to 480.86±3.98 , 496.70±3.08 ,522.18±3.19 respectively(P<0.01).Conclusion Salidroside dose-dependently increased [Ca²⁺]I in cardiomyocytes, at least partly due to released from sarcoplasmic reticulum via RyR2 Ca²⁺ channel in Cultured Rat.