The Diversification Of Atrial Myocytein Action Potential (AP) In Patients With Atrial Fibrillation

Objective: To research the changes in ion channels by investigate the diversification on action potential duration (APD) in patients with atrial fibrillation (AF).Methods: 1.Modified enzyme digestion with acute isolation of adult atrial.2. Smoking a few drops of the suspension of prepared ventricular myocytes were added to cell pool, and placed in bio-inverted microscope, table, sink to the bottom adherent cells, the red to the dead cell debris, select the rod-shaped, smooth surface, Clear texture study of myocardial cells;3. The use of mobile electrodes in three-dimensional manipulator inverted microscope, select the plasma membrane integrity, stripes and a clear heart muscle cells;4.Site drawn glass microelectrodes, electrode filling 3mol / L of saturated KCI solution;5. Through the patch clamp technique seals, rupture of membrane capacitance and series resistance compensation for whole-cell patch clamp recording the formation of the state.6. By using the current clamp technique, with the EPC-10 stimulator output square wave (pulse width 2ms frequency 1Hz1.5 times the threshold intensity voltage) driver samples, leads to a single atrial cell AP. During the experiment by the software pCLAMP 10.0, amplifiers, digital to analog converter to complete the generation of stimulatory signals, feedback signal acquisition and storage. After a 1 kHz input signal filtering, data stored in the computer hard drive.7. Laboratory temperature should be maintained at 20-25℃.8. Records of adult atrial myocytes isolated AP, atrial fibrillation group compared with the normal group differences in APD of atrial muscle cells9.The statistical data are ( X|-±s), said statistical software with SPSS17.0 treatment groups were compared using independent sample T test, P <0.05 indicated that statistically significant.Results: APD,action potentials at 50% and 90% of repolarization (APD₅₀, APD₉₀) were significantly shorter than that in the controls (P<0.05 ).Conclusion: The cut down of APD was related to the changes of ion channels in atrial myocyte, in particular those which can affect the action potential repolarization, such as changes in potassium and calcium channels which affect the action potential repolarization.