Study On STZ Treatment To Tree Shrews Beta Cell Regeneration And EGF, IGF-1 Expression

Objective:Establishment of tree shrew model of pancreaticβcell injury, confirmed that the tree shrew isletβ-cells regeneration and the relationship with EGF,IGF-1 expression.Methods:Feeding the environment will adapt the 32 adult tree shrews were divided into two groups , including the 28 STZ treated group and control group 4.2 weeks to STZ(each 120mg/kg) injected a total of three times and then observed for 10 days,Were divided into treatment group A according to blood glucose( normal blood glucose)5,treatment group B(increased blood sugar returned to normal after the first)8,treatment group C(blood glucose increased slightly but less than diabetes standard)6,treatment group D(diabetic group) 5.E group,control group 4 .Control group received intraperitoneal injection of acidified saline 5ml/kg.STZ3 times after injection 3d,1w, 10d detection Blood glucose,weighed,Before the injection and death of insulin, and calculate Insulin sensitivity index. Pancreatic specimens collected,Observed under light microscope morphological changes of islet,HE staining of isletinflammation,Insulin was detected by immunohistochemistry, Measurement of islet Tissue cell regeneration of factor Pdx-1, Ngn3, EGF, IGF-1 expression.Results:(1)A group, B group remained normal islet morphology, cell volume compared to normal, relatively full island-shaped, C group had a small amount of the cytoplasm of islet degeneration, D group significantly reduced the number of pancreaticβcells, islet empty.(2)Compared with the D group, B group tree shrew islet Expression of INS was significantly higher (P <0.05),B group tree shrew increase in serum insulin levels, glucose levels decrease.(3)STZ treated group ISI was significantly lower than the control group (P <0.05); with the D group, A, B, C group tree shrew ISI were significantly higher (P <0.05), where A, B group and C group significant difference, ISI increased more significantly (P <0.05), A, B did not differ between groups (P> 0.05).(4)STZ treatment of PDX-1 and Ngn3 protein antigen lower than the control group (P <0.05); compared with C group, D group B group was significantly higher (P <0.05),There was no difference with the A group (P> 0.05).(5)STZ treatment of EGF and IGF-1 protein antigen lower than the control group (P <0.05); compared with C group, D group B group was significantly higher (P <0.05),There was no difference with the A group (P> 0.05).Conclution: STZ treated tree shrew islets may be expressed through the promotion of Pdx-1, Ngn3, EGF, IGF-1 and other related factors For the Tree shrew experimental Diabetes residualβ-cell proliferation and renewable play a role in promoting. Improve glucose metabolism and insulin resistance, and to some extent, promote the recovery of islet function and lower blood sugar.