A Primary Study On The Interaction Of Calcifying Nanoparticles With Human Renal Tubular Epithelial Cells

Objective To observe the interaction of the calcifying nanoparticles (CNP) with human renal tubular epithelial cells (HK-2) in vitro. To investigate the mechanisms of HK-2 damage incited by CNP, and to investigate the potential role of CNP in the formation of nephrolithiasis.Methods Human renal tubular epithelial cells were cultured with CNP in vitro, and the morphology changes was observed by light microscopy and electron microscopy. To investigate the oxidative stress, NADPH oxidase inhibitor apocynin was chosed as the intervention treatment. The levels of LDH, MDA, HA in the mediums and the activity of NADPH oxidase in human renal tubular epithelial cells were assessed after 24 hours. The expression of NADPH oxidase subunit p22phox and p47phox mRNA were detected by real-time quantitative PCR (RT-PCR).Results CNP can cause morphology changes of the HK-2, have observed the adhesion and phagocytosis of CNP by the HK-2 under TEM. After 24 hours, the level of LDH, MDA, HA were significantly different among the 4 groups (P <0.05).Conclusion HK-2 has abilities of adhering and phagocyting with CNP; and CNP can cause the damage induced by oxidative stress of HK-2.