The Research On Screening Different Genes In Invasive Ductal Carcinoma Of The Breast By Using Micro Array

Objective: To study the differentially expressed genes in human invasive ductal carcinoma(IDC) and normal tissue of the breast by using microarray, and establish the gene expression profiles of IDC.Methods: In the period of March 2010 to August 2010, considered estrogen receptor (ER) positive, progesterone receptor (PR) positive, human epidermal growth factor 2 (HER-2) negative, and lymph node metastasis as the standard, collected 3 specimens of pathologically confirmed cancer tissue and adjacent corresponding normal tissue from department of Gastrointestine and Gland Surgery the First Affiliated Hospital of Guang Xi Medical University to extract total RNA. Reversely transcribed synthesis of cDNA, by in vitro transcription of the 6 specimens of cDNA into cRNA probes labeled respectively to use Cy3 fluorescence, and hybridized to the six microarrays of Human WG-6 expression beadchip which contains 48000 genes and ESTs (Expressed Sequence Tags). Scan for the fluorescent signals by using fluorescent scanner and analyze differences in gene expression by importing datas to GeneSpring11.0 analytical software. Select differentially expressed genes between IDC and normal tissue, and make a bioinformatics analysis of them.Results: According to fold changes≥2.0 times, or≤0.5 times as the differentially significant standard, 3 pairs of specimens have a common differentially expressed genes of 6756, accounting for the number of total microarray transcripts of 14.08%, the expression of 3927 of which were up-regulated and the expression of 2829 of which were down-regulated in the 6756 genes. If that significant difference in the standard is raised to 10 times, then 3 pairs of specimens have a common differentially expressed genes of 562, the expression of 313 of which were up-regulated and the expression of 249 of which were down-regulated in the 562 genes, including oncogenes and tumor suppressor genes, DNA synthesis and repair genes, immunity genes, cell signaling and transduction of genes, protein synthesis and translation of genes, metabolism genes, cell cycle genes, apoptosis genes, and some genes of unknown function.Conclusion: (1)Succeed in establishing gene expression profiles of IDC initially. (2) In tracking through biological information of different genes, suggest that occurrence and development of IDC have multiple changes in gene expression, microarray technology can study a large number of quantitative gene expression levels at the same time, which is beneficial for understanding the pathogenesis of tumor and to provide biological basis for individual treatment.