Experimental Study On Grape Seed Procyanidin Against Human Pancreatic Cells

Objective To investigate the inhibiting effect of different concentration gradient GPCand with different time point, on the pancreatic cancer cells(BXPC-3) in vitro, and to research itspossible mechanism.Methods Choose different concentration GPC(0μg/ml,50μg/ml,100μg/ml,200μg/ml)which get after doing experiments in advance,Acting on BXPC-3 respectively, At thepoint(24h,48h) in time, different concentration GPC influence on cells proliferation by usingMTT assay. In the most appropriate time,the optimal concentration of GPC according to MTTassay act on cells,Detect the expression levels of Bax,Bcl-2,Cox-2 by immunohistochemicalmethod- SABC. To detect the effects of GPC on the cell cycle by means of FCM (Flowcytometric profilometry ).Results1 MTT assay : After using a certain amount GPC intervention BXPC-3 cell lines that weregenetically normal,Each group the cells' growth have been suppressed. In dosing concentrationfor 200μg/ml act when 48h, The inhibition rate of cell proliferation is 81.5%, Inhibition of cellproliferation is the most obvious.2 Immunohistochemical method: The GPC(0μg/ml,200μg/ml) act BXPC - 3 cells with24h, can enhance the expression level of Bax, reduce the expression levels of Bcl-2,Cox-2, Halfquantitative analysis reveal a statistically significant difference between groups(P﹤0.01).3 Flow cytometry: The results of FCM show that, Different doses of GPC act on cell,along with the GPC doses of increase, G₀ / G₁ phase cell progressive increase, but S period, G2 /M period cells gradually decrease.Conclusion1 It show that the GPC inhibit the proliferation of BXPC-3 with obvious relationship oftime- effect and dose -effect in vitro.2 The possible mechanism of GPC inhibiting cell proliferation relate to Reducing Bcl - 2,Cox - 2 expression levels and enhancing Bax expression level.3 The GPC can make BXPC-3 cell stagnation in G₀ / G₁ phase...