Associativity Study Between H-CD Degradation Regularity And The Estimation Of Postmortem Interval In Stomach And Appendix Of Mice

Objective:According to observe the postmortem degradation of high molecular-weightcaldesmon(h-CD)in stomach and appendix smooth muscle cells ofmice by Western Bloting,study the erects ofpostmortem intervals,and ambient temperature on the h-CD degradation ofmice,and the variability of the h-CD degradation in stomach smooth muscle cells and appendixsmooth muscle cells in different ambient temperature．Evaluate the application for estimatingthe time ofdeath in forensic medicine aspect．Methods:72 healthy adult Kunming mice were killed by asphyxia,and put them in 4℃refrigerator and 25℃artificial climate incubator respectively,then extract the stomach andappendix tissues at Oh,24h,48k72h,96h,120h after dead．The h-CD contents in the abovetissues were quamtitated by western-blot assay and collected by Image Pro Plus 5．0 imageanalysis system,then the datas were statistically analyzed with the SPSS 16．0 soflwaxe．Results:(1)After death,H-CD content in stomach and appendix tissues decreased with thepostmortem intervals,there is correlation between the postmortem degradation of h-CD and thepostmortem intervals;(2)The differences of the postmortem degradation of h-CD in different ambienttemperature were significant(P<0．05,．the degradation in 25℃faster than 4℃．The multipleregression equation between actin content and ambient temperature,PMl were:at 4^C stomachtissues Y=14547．324-2574．543X,R₂=0．997．appendix tissues Y=13326．062-2587．111X,R₂=0．963;At 25℃stomach tissues Y=13917．886-2878．454X,R2=0．962．appendix tissuesY=11939．029-2663．071X,R2=0．870(Y was the IOD value of the h-CD content．X was thePMI,)(3)The differences of the postmortem degradation of h-CD between stomach andappendix tissues were significant(P<0．05),the degradation in the appendix more faster．ConelusionH-CD content in stomach and appendix tissues decreased gradually with the postmortem intervals,the postmortem degradation ofh-CD was influenced by ambient temperature,and thedegradation rate tended to be raised with higher temperature,and the differences of thepostmortem degradation of h-CD between stomach and appendix tissues were significant,thedegradation in the appendix more faster．The above analysis may be used for the estimation ofpostmortem intervals in forensic practice．...