| Bjective: To study the expression of connexin 43,32 in the hippocampal tissue of epilepsy—rats induced by pilocarpine and the effects of carbenoxolone on them,and investigate the role of connexin 43,32 in epileptogenesis and the antiepileptic mechanism of carbenoxolone.Methods : SD rats were randomly divided into control group,pilocarpine group and carbenoxolone group respectively.The experimental epilepsy model of the kindled rat was intraperitoneal injuction of lithium-pilocarpine. Rats in pilocarpine group and carbenoxolone group were dicided into six subgroups after seizure for 3h,6h,12h,24h,3d and 7d,respectively. One houre before pilocarpine were injucted,rats in carbenxonlone group were pretreated by intraperitoneal injuction of carbenoxolone with a density of 20mg/kg according to body mass. The control group were raised without any special treatmeat. The epilepsy rats induced by pilocarpine and that treated by carbenoxolone were assessed by Racine score and their latency of seizure was recorded. Observed morphological changes of hippocampus by HE staining. The expression of connexin32 and connexin43 in the hippocampal was measured by immunohistochemistry. Results:The rats in control group were seizure-free,no different in the latency of epileptic seizure between pilocarpine group and carbenoxolone group(P>0.05).HE staining show, 6 hours after seizure, the cell shrinkage,drop out began to appear in hippocampal in pilocarpine group, especially in CA1,CA3 and detate gyrus,and the seventh day most obvious;the carbenoxolone group also experienced this phenomenon, But the degree of neuronal loss lighter than the model group.The expression of Cx32,Cx43 was increased significantly in pilocarpine group and carbenoxolone group compared with the control group(P<0.05), especially on the 24th hours after seizure,and decrease later; Within 24 hours after seizures, no different in expression level between pilocarpine group and carbenoxolone group(P>0.05),but there were significant different in 3th day group and 7th day group(P<0.05).Conclusion: 1.The Cx32 and the electrical synapse make up of Cx32 between neurons involved in the development of epilepsy; 2.The expression of Cx43 was increased significantly after seizure indicated that the links between neurons and astrocytes more closely,and Cx43 expression increased both the result of epilepsy, but also a contributing factor to the development of epilepsy maybe; 3.The Carbenoxolone did not show significant protective or therapeutic effect in the acute stage,but in the stationary phase, CBX showed obvious protective effect of neuronal.
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