The Effection Of Pulmonary Fibrosis On Erectile Function In Rats

Object:study the effection of pulmonary fibrosis on erectile function in rats and discuss the mechanism. Methods:40 healthy malel2-week-old Sprage-Dawley rats were randomly devided into 4 groups: pulmonary fibrosis group 4 weeks(A group) and 6 weeks(B group), control group 4 weeks(Cgroup) and 6 weeks(D group), respectively at 4 weeks and 6 weeks all rats underwent erectile function testing by measuring intracavernosalpressure/meanarterialpressure(ICP/MAP),serumtestosterone and PO2 were detected, penis were ectomized, NOS activity and cGMP Content were examined,eNOSmRNA,iNOSmRNA andnNOSmRNA were detected by real-time PCR, eNOS was analyzed by Westernblot。Results:The ICP/map*100 in group C,3v,5v(16.37±2.19,27.19±3.18) was significantly less than in group A (30.78±2.66,50.09±6.97) (P<0.05), The ICP/map*100 in group D,3v,5v (10.17±1.31,17.40±1.74) was significantly less than that of group B (31.45±3.07,51.23±7.23) (P<0.05), and group D was significantly less than that of group C (P<0.05). The PO2 in group C(75.50±13.87)mmHg was significantly less than in group A(103.80±6.88)mmHg(P<0.05), group D(83.60±5.50)mmHg was significantly less than that of group B(102.70±5.77)mmHg (P<0.05). serum testosterone in group C (391.08±264.65) ng/dlwas significantly increased than in group A(175.88±52.97)ng/dl (P<0.05),group D(745.43±408.84)ng/dl was significantly increased than that of group B(177.79±52.34)ng/dl (P<0.05) and group D was significantly increased than that of group C (P<0.05). the NOS activity and cGMP of corpus cavernosum in group C (1.502±0.140U/mgprot, 35.694±3.638 pmol/mg) was significantly less than in group A(2.656±0.389U/mgprot,51.103±7.220 pmol/mg) (P<0.05), group D (1.396±0.199 U/mgprot,34.554±4.304 pmol/mg) was significantly less than that of group B (2.745±0.356 U/mgprot,52.152±6.855 pmol/mg) (P<0.05),and group D was no significant difference with group C (P>0.05). the expression of eNOS in group C (0.79±0.01) was significantly less than in group A(0.87±0.01) (P<0.05), group D(0.71±0.02) was significantly less than that of group B(0.88±0.01) (P<0.05) and group D was significantly less than that of group C (P<0.05). the expression of eNOS mRNA in group C(4.46±0.92) was significantly increased than that of group A(2.61±0.68) (P<0.05), group D (2.79±0.60)was no significant difference with group B(2.69±0.65) (P>0.05),and group C was significantly increased than that of group D (P<0.05),the expression of nNOS mRNA and iNOS mRNA in pulmonary fibrosis group was no significant difference with control group (P>0.05). Conclusion:pulmonary fibrosis may mediate erectile dysfunction through the expression of eNOS in corpus cavernosum of rat, Simultaneously decrease the activity of NOS and the Content of cGMP.