Interaction Of Protein-Protein Interaction Of Hemagglutinin(HA) And Neuraminidase(NA) On Surface Of Influenza A Virus

Influenza A is ball-like virus and covered with lipid molecule. Hemagglutinin (HA) and neuraminidase (NA) are distributed on its surface. Generally, most researchers hold the opinion that HA would bind with sialic acid(SA) on the cell surface in the early stage of infection so that the virus could entry the host along with endocytosis bubbles, and after production of new virus, NA could cut down the HA-SA binding and let the virus release from the host cell. In recent years, some experimental phenomena show that NA would affect the function of NA during the entry period, however, limited by experiment technology of protein-protein interaction, no one has study on interaction sites between HA and NA.In this thesis, distance matrix and correlation coefficient were used to compare the evolution process of HA and NA. The result showed there was close functional relationship between two proteins. Then we divided the sequences into segments and calculated the correlation coefficient between different residues to search for the interface of HA-NA complex. Finally, we applied the molecular dynamics simulation to study the process of interaction and computed the binding energy on sites. The result proved the predicted results were reliable.