| Grifola frondosa, belonging to the family Polyporaceae, order Aphyllophorales, has multiple bio-activities. Our lab has isolated three compounds from Grifola frondosa, They are 5α,8β-epidioxyergosta-6,22 diene-3(3-ol, ergosterol & 3β,5α,6p-trihydroxyergosta-7,22-diene.Carpesium faberi Winkl, belonging to Compositae, is rich in various kinds of sesquiterpenes. Studies have indicated sesquiterpene compounds have several bio-activities including anti-tumor and anti-angiogenesis. We obtained two sesquiterpene compounds,4-epi-isoinuviscolide & 8α,15-dihydroxy-13E-labdene, isolated from Carpesium faberi Winkl by Laboratory of Brain Functional Genomics.MTT assay and flow cytometry were used to determined the inhibition activity and apoptotic activity of these compounds on MCF-7. It was found that these five compounds could suppress the proliferation of cancer cells variously.5a,8β-epidioxyergosta-6,22 diene-3β-ol,4-epi-isoinuviscolide and 8α,15-dihydroxy-13E-labdene significantly inhibited the proliferation of MCF-7. The IC50 of these three compounds are 53.9μmol/L,68.6μmol/L and 59.2μmol/L. The flow cytometry result show that all these three compounds block the MCF-7 cell cycle in G2/M and promote apoptosis of MCF-7.Based on the results on MCF-7 cells, we use MTT assay, cell scratch assay, trans well assay and tube formation assay to demonstrate that 5a,8(3-epidioxyergosta-6,22 diene-3(3-ol,4-epi-isoinuviscolide and 8a,15-dihydroxy-13E-labdene could inhibit vascular endothelial growth factor (VEGF) induced cell proliferation, migration and capillary-like tube formation of human umbilical vascular endothelial cells (HUVECs) in vitro. The result of chorio allantioc membrane (CAM) assay indicates that these compounds also have anti-angiogenesis effect in vivo.
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