Effects Of Rhamonolipids And LPS On The Adhesion Of Pseudomonas Aeruginosa At PEG-SAMs Surface

Poly (ethylene glycols) has been used as a surface coating to prevent adsorption of protein and adhesion of bacteria as well as the cells in buffered aqueous solution to formed the biofilms. The coating of PEG brush yielded a large degree reduction in bacterial adhesion of the most bacteria have been studied except Pseudomonas aeruginosa, so it is a good chance of explanation the mechanism of antifouling of PEG-SAMs to answer the question about which molecules take play on the process of P. aeruginosa adhere on this surface. In this studie, I have screened the non-adhesive strains of P. aeruginosa as control strains for further work, and using flow chamber to test the strains of expressing different types lipopolysacchrides(LPS) and rhl genes knock-out strains initial adhesion rate for distinguishing the role of LPS and rhamnolipids in process of P. aeruginosa adhesion on the PEG-SAMs. I have used Au/EG3-SAMs nanoparticles to isolate the adhesive components on P.aeruginosa outer membrane. Finally, we find that the LPS and rhamnolipids play no role in the process of P.aeruginosa adhere on PEG-SAMs.