| Background:Gastric motility of patients underwent abdominal operation were easily subject to stimulation and injuries by anesthetization and surgery, long-time exposure of gastrointestinal tracts and possible infections which will lead to disorders of gastrointestinal neural reflexSymptoms like abdominal distention and pain irritate patients, compress inferior vena cava, and impede healing of incisions. Early recovery of gastrointestinal function is the key to shorten hospital stays.Objective:To study the regulation and mechanism of acupuncture on gastric motility and gastrointestinal peptides of abdominal post surgery.Methods:1. Grouping:SD rats were randomized into 4 groups.Model group:received enteroenterostomy. Underwent anesthesia by intra-peritoneal injection on 10% filtered chloralic hydras (0.33ml/100g), then fixed in supine position, through routine skin preparation, sterilization and draping. Created a 2cm long incision along the median on the lower abdomen, exposed the cavity, located colon 2cm below cecum, excised and sutured in situ (single-layer anastomosis), applied running suture on peritoneum and muscular layer and interrupted suture on skin. No treatment was given after surgery.Sham operation:Removed stomach, small intestine and partial colon in vitro, and covered with carbasus infiltrated by warm saline, replaced 30min later, then closed the incision. Acupuncture was given post-surgery.Acupuncture group:received enteroenterostomy. Acupuncture was given post-surgery.Blank group:No surgery, no treatment.2. Observation:2.1 Radionuclide scanning on gastric emptying Fasted 12 hours before tests on the third day, intra-peritoneal injected diazepam (4mg/kg).Placed rats into a cylinder fixator, offered liquid test meal (mixture of 99mTc-DTPA and 0.9% NS, at dose of 0.1-0.2mCi99mTc-DTPA/1.5ml test meal/rat). Settled fixators on rats'vertical position on the test bed, adjusted the probes of collimator to focus on abdomens, underwent sequential dynamical acquisition at energy peak of 160keV, window width of 20, matrix of 256x256, zoom of 1.0, acquisition time of 20s/frame, every 30min for pre-surgery, every 50min for post-surgery. Data were processed by software (GASTRICEMPTYING) to create a gastric emptying-time curve, in which we located GET1/2 and gastric emptying rate of 60min.Time t gastric emptying %=(total radiation-t radiation K)/total radiationx100%K is the radioactive decay correction coefficient from time 0 to time t. (Auto-processed by computer)2.2 Plasma MLT, gastrin, somatostatin, vasoactive peptideFasted with free access to water for 24 hours, sampling blood of 2ml after decollation. Blood was mixed with 30uL 10% EDTA·Na2 and 40uL aprotinin, quiescent in 4℃refrigerator for 1-2hours followed by centrifugation at 3000 rpm for 10min. Supernatant was preserved in-20℃refrigerator. Supernatant was processed with RIA, strictly followed the instruction of kit, tested by pre-programmed automatic gamma-ray counting system to obtain related parameters.Results:1. Radionuclide scanning on gastric emptyingData of GET1/2 and emptying rates of 60min between model group and blank group showed significant difference (P<0.05), while the difference was more remarkable between acupuncture and model group (P<0.01). No significant difference was concluded in comparison of acupuncture and blank group, as well as in sham operation group (P>0.05). sham operation group and blank group were also be proved by the non-significant difference (P>0.05). However there was significant difference noticed between model and sham operation group (P<0.05)2. Plasma MLTSignificant differences were seen between model group and blank group as well as in acupuncture and model group (P<0.01). No significant difference was concluded in comparison of acupuncture and blank group, (P>0.05). sham operation group and acupuncture group were also be proved by the non-significant difference (P>0.05).However there was significant difference noticed between model and sham operation group (P<0.01)3. gastrinSignificant differences were seen between model group and blank group as well as in acupuncture and model group (P<0.01). No significant difference was concluded in comparison of acupuncture and blank group, (P>0.05). sham operation group and acupuncture group were also be proved by the non-significant difference (P>0.05).However there was significant difference noticed between model and sham operation group (P<0.01)4. somatostatinSignificant differences were seen between model group and blank group as well as in acupuncture and model group (P<0.05). No significant difference was concluded in comparison of acupuncture and blank group, (P>0.05). sham operation group and acupuncture group were also be proved by the non-significant difference (P>0.05).However there was significant difference noticed between model and sham operation group (P<0.05)5. vasoactive peptideSignificant differences were seen between model group and blank group as well as in acupuncture and model group (P<0.05). No significant difference was concluded in comparison of acupuncture and blank group, (P>0.05). sham operation group and acupuncture group were also be proved by the non-significant difference (P>0.05).However there was significant difference noticed between model and sham operation group (P<0.05)Conclusion:Acupuncture can improve the gastric emptying after abdominal operation. The mechanism may be related to the regulation of acupuncture on secretion of plasma MLT and gastrin, somatostatin, vasoactive peptide. |
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