The Expression Of GRP78 And Caspase-12 In Rats Sleep Deprivation During High-altitude Environment And Medicine Intervention

Objective:1.To investigate the dynamic expression of GRP78 and Caspase-12,neuron apoptosis in the rat hippocampus on different stages of REMSD in plateau.2.In the high altitude By offering medicine we observed the effect of GS-Rd on the expression of GRP78 and Caspass-12 in the rats hippocampus in the model of sleep deprivation. To investigate the possible neouruprotective effect of Ginsenoside Rd.Methods:The Gan Su medical college animal experiment center offered the 140 wistar rats which body weight from 180 to 300g were distributed randomly into the experiment.the wistar rats were distributed randomly into two groups:Lan Zhou group and KeKeXiLi group; and each group was distributed into normal sleep (n=5)and GS-Rd group(SD1d,3d,5d) and normal saline group(SD1d,3d,5d) again, after rejected rats that drowning or accidental death,5 rats per time point were accomplished randomly.At the experiment SD was induced in wistar rats by "flower pot " technique. We Check out expression protein of GRP78 and Caspase-12 with Immunohistochemistry staining. And check out apoptosis cell with TUNELResults:Immunohistochemistry staining results showed the expressions of GRP78 and Caspase-12 protein increased after one day of REM sleep deprivation compared with those in rats with normal sleep in Lan zhou groups. and reached the highest on the third day.while on the fifth day, the expressions of GRP78 and Caspase-12 protein were not different from those of normal sleep group. After intervention of Ginsenoside Rd, the expression of GRP78 were higher than physiological saline groups after first or third day of REM sleep deprivation. The expression of GRP78 protein were not different from physiological saline groups on the fifth day. And after intervention of Ginsenoside Rd,the expression of Caspase-12 were lower than physiological saline groups after first or third day of REM sleep deprivation. The expression of Caspase-12protein were not different from physiological saline groups on the fifth day. At the same time the expression of GRP78 and Caspase-12 protein were higher compared with Lan zhou group in the Ke ke xi li group.Conclusion:1.After Endoplasmic Reticulum stress (ERS),organism basis homoiostasis system was primed,but long-term and severe Endoplasmic Reticulum stress can destruct this basis regulation,and it primed apoptosis pathway,this is maybe one of these mechanisms that sleep deprivation injured brain tissue; 2.High altitude aggravate further Endoplasmic Reticulum stress; 3.GS-Rd can increase the expression of GRP78 and decrease the expression of Caspase-12 to lessen Endoplasmic Reticulum stress,so that,brain tissue was protected.