Methylation Status Of Promoter Of SPARC In Epithelial Ovarian Cancer And Clinical

Objective To study the role of promoter hypermethylation of SPARC gene in the course of tumorigenesis and development in epithelial ovarian carcinoma.Methods The methylation status of promoter region SPARC was assessed by methylation-specific polymerase chain reaction (MSP) in formalin fixed, paraffin embedded tissues from 40 pithelial ovarian cancer patients,30 benign ovarian tumor patients and 10 normal ovarian patients.Results The methylation frequency of SPARC was (26/40) 65.0%, (8/30) 26.7% and (2/10) 20.0% in ovarian cancers, benign ovarian tumors and normal ovarian tissues respectively. Between the different organization type The hypermethylation of SPARC gene was more frequently encountered in ovarian cancers than in tumor and normal tissues (P<0.05). There was no diference between tumora and normal tissues, and also between the different organization type(P>0.05). The hypermethylation frequency of SPARC gene in poorly differentiated ovarian cancer tissues was higher than well-differentiated and moderately tissues,and significant (P<0.05). There were not significant between moderately differentiated and well-differentiated tissues. The frequency of promoter hypermethylation of SPARC was significantly lower in epithelial ovarian cancers of stageâ… andâ…¡than that in stageâ…¢and (P<0.05), hydroperitoneum group was higher than that without hydroperitoneum (P<0.05).But there was no difference between lymph node metastasis and no lymph node metastasis (P>0.05)Conclusion:The mrthylation of of promoter hypermethylation of SPARC gene has been postulated to play an important role in the development of ovarian cancer.This methylation correlates with histopathologic grade and clinical stage of epithelial ovarian cancer.