A New Mechanism Of LPC Promoting Atherosclrosis

LPC is physiological active lipid included in Lipoprotein, it will be 30-50 times higher in ox-LDL. LPC is main active lipid and function medium of ox-LDL. For instance, LPC stimulate Proli-feration and migration of smooth muscle cells. The function promote arteriosclerosis. Mechanism which LPC induce proliferation and migration of smooth muscle cells is very important to occurre-nce and development of arteriosclerosis and treatment.In this study, human aortic vascular smooth muscle cells (hAoSMCs) make model, LPC promotes signal transduction of cell migration. LPC which is under phospholipase D or autocrine motility factor (Autotaxin ATX) hydrolyse to LPA and stimulate migration of smooth muscle cells by receptor signaling pathways. The process of cell migration promoted by LPA Inhibited LPA rec-eptor antagonist (Kil6425). Gi protein inhibitor (PTX), ERK1/2 inhibitor (PD98059). P38 inhib-itor (SB203580). Through the production of animal models of atherosclerosis (snow rabbit), we found that content of plasma LPC and the activity of phospholipase D increased in varying degrees. Expression of Autotaxin and LPA1 receptor gene increased in model group. Again, it proved there is closely relate with the improvement and atherosclerosis when increased of LPC content, expression of Autotaxin and LPA1 receptor and activity of Autotaxin or phospholipase D.Conclusion (1) Signal transduction pathways of LPC promote cell migration is the LPC Autotaxin (phospholipase D)-LPA-LPA1-Gi-P38 and ERK in vascular smooth muscle cells. (2) Atherosclerosis animal model experiments indicate that, there is closely relate with the improvement and atherosclerosis when increased of LPC content. expression of Autotaxin and LPA1 receptor and activity of Autotaxin or phospholipase D.