Study On Hypoglycemic Mechanism Of Gegen Qinlian Decoction Experimental Diabetic Animal Modle

This study is investigated the hypoglycemic and hypolipidemic of Gegenqinlian Decoction (GQD).The antioxidant activity of GQD is also measured through the total antioxidant activity and organic free radical (DPPH·) scavenging. By using LC-MS method, endogenous metabolites are analysised the difference in urine between normal animals and diabetic animals.And the data is analysised through the principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) methods, in order to look for the potential diabetes biomarkers, and develop diabetes early diagnosis methods based on metabonomics and new methods of drug screening.The hypoglycemic effect of Gegen Qinlian Decoction (GQD) on alloxan induced diabetic model rats and STZ induced diabetic model mice was studied. The effect of hypolipidemic, antioxidant activity and the hypoglycemic mechanism of the decoction were evaluated. The diabetic mice were induced by the intravenous injection of alloxan (ALX). 15d after administration, the blood glucose, serum triglyceride (TG) and high density lipoprotein (HDL-C) were determined since 1 h after the last administration. The effect of antioxidation of GQD and each prescription herb was determined by Ferric reducing antioxidant power (FRAP) assay and 2,2-diphenyl-1-picryl-hydrazyl (DPPH·) free radical-scavenging assay, respectively. The diabetic rats were induced by the abdominal injection of STZ. 20 d after administration, the blood glucose was determined since 1 h after the last administration.GQD could significantly reduce the blood glucose levels of alloxan-induced diabetic mice and STZ-induced diabetic rats, and decreases levels of serum TG and increases HDL-C levels. In addition, GQD has good antioxidant activity. GQD has significantly hypoglycemic effect. By the effect of hypolipidemic and antioxident, GQD plays a integral role in treatment of diabetes. In addition, the Scutellaria plays a leading role in the whole effect of antioxidation of GQD. The urine of STZ-induced diabetic rats was detected by LC-MS analysis to establish metabolic graphy. The mobile phase was included acetonitrile (containing 0.1% formic acid) and ultra pure water (containing 0.1% formic acid) for gradient elution. 393 compounds of the blank group and model group, 354 compounds of model and Gegenqinlian Decoction group were detected through SIEVE software for SCORE greater than 0.6 standard. Then, detected compounds were analysised by using PCA and PLS-DA, control group and model group received 104 compounds, model and Gegenqinlian Decoction group got 164 compounds. Accrording to the m/z and retention time, the same 31 potential biomarkers were found from the 104 and 164 compounds. Compared to the HMDB and ChemSpider databases, the m/z197 compound was identified.And it was sured D-gluconic acid through the retention time, molecular weight and MS/MS spectra. In the urine, it showed a trend to the model group from the control group was increased, and a trend to the model group to Gegenqinlian Decoction group was decreased. Thus, Gegenqinlian Decoction has a certain role on diabetes prevention and treatment by the analysis of metabolomics approach.