The Expression Of FOXP3~+ Treg And IL-10 And Correlation To Prognosis In DLBCL

Objective To investigate the expression of FOXP3+Treg and IL-10 in diffuse large B-cell lymphoma (DLBCL) , detect its clinicopathological significance and explore the relationship between FOXP3+Treg and IL-10 and prognosis and clinical feature. Methods The absolute number of FOXP3+Treg and IL-10+ cells was immunohistochemically studied from 69 patients of DLBCL and 26 cases of normal tonsils and lymph nodes and correlated to phenotypic and clinical parameters. Using T test to make pair comparisons. Using ANOVA to contrast grouped comparison. ROC curves were used to determine prognostic cut-off values of FOXP3+Treg and IL-10+ cells density. Overall survival rates were analyzed by the Kaplan-Meier method applying cut-off values determined by the ROC/Y and primary dichotomized clinical characteristics. Results The absolute number of FOXP3+Treg cells and IL-10+cells was significantly different between that in normal tonsils and lymph nodes and in DLBCL (P<0.01). FOXP3 was expressed in interfollicular and mantle cell in normal tonsils and lymph nodes, the absolute number was (35.3±11.4)/mm2 and the CV was 0.32. FOXP3 diffuse in DLBCL and the absolute number of intratumoral FOXP3+ cell was (55.7±40.5)/mm2 and the CV was 0.73. IL-10 was expressed in interfollicular and mantle cell in normal tonsils and lymph nodes, the absolute number was (51.3±13.1)/mm2 and the CV was 0.43. FOXP3 diffuse in DLBCL and the absolute number of intratumoral FOXP3+ cell was (79.0±39.8)/mm2 and the CV was 0.50. The expression of FOXP3+Treg had no relationship to gender(P=0.170), age(P=0.120), clinical stage(P=0.377), pathological subgroup(P=0.339), biopsy site (P=0.996), ENI(P=0.996) and therapy(P=0.804). The expression of FOXP3+Treg had relationship to LDH(P=0.019). The expression of IL-10+ cell had no relationship to gender(P=0.692), age(P=0.402), clinical stage(P=0.144) , pathological subgroup(P= 0.565), LDH (P=0.577), biopsy site (P=0.357), ENI(P=0.357) and therapy(P=0.963). The expression of FOXP3+ Treg cell and IL-10+ cell had no relationship to short-term outcome. The expression of FOXP3+Treg cells in non-GC DLBCL and IL-10+ cells in GC-like DLBCL was correlation to prognosis(P<0.05). The expression of FOXP3+Treg cells in GC DLBCL and IL-10+ cells in non-GC-like DLBCL was not correlation to prognosis(P>0.05). Cox regression analysis proved that stage, FOXP3, subtype were independent factors for DLBCL, but IL-10 was not. Conclusion FOXP3 and IL-10 diffused in background of DLBCL tissues and represent important lymphoma/host microenvironment modulators. The expression of FOXP3+Treg and IL-10 cells serves as a prognostic indicator of DLBCL.