| Objective Coronary heart disease is still frequently occurring in our country, and coronary artery bypass vein accompanied by the process of arterial revascularization. C - reactive protein (CRP, acute phase reactants) an important inflammatory factor and a good independent predictor of coronary syndrome, the degree of vascular response to injury. C-reactive protein (CRP) is a sensitive inflammatory marker in systemic and local inflammatory response t. C reactive protein was found in the process of autologous vein graft arterie and is closely linked with atherosclerosis, However, early in the process of vein graft and wheather involved in the process of intimal hyperplasia has not been fully confirmed However, the study of between CRP and transplantation of autologous vein grafts after arterialization is still less. This study aimed to investigate the mechanism (s) upregulation of CRP in the vein graft arterialization。And from conduct the body of the jugular vein in rat artery model to simulate the physiological process of CABG surgery, application of highly selective p38MAPK inhibitor CBS3830 intervention, and reasearch the changes of CRP, discuss the role and significance of CRP in the process of the vein arterialization ,try to reveal the role of the CRP in the process of the vein arterialization after vein graft, to further provid a new way for prevent the bridge blood vesselso restenosis and intimal hyperplasia .Methods 70 SD rats were randomly divided into four groups: control group (n=20), sham-operated group (n=10), single doses group (n=20) and double doses group (n=20). Sham-operated group, only simulated surgical procedure,without vascular grafts and drug intervention; control group, jugular vein to carotid artery, and given the solvent (Solvent, methyl cellulose) 2h before transplantation once, 3mg/kg intervention; sigle doses group only once,3mg/kg(CBS3830), 2h before transplantation;double doses group twice, 3mg/kg(CBS3830) per time, 2h before transplantation and 4d after transplantation. Taking blood samples at time points of 0h, 24h, 48h, 4d, 1w and 2w.. Enzyme-linked immunosorbent assay (ELSIA) was used to detect the serum levels of crp. HE staining was used to detect the pathological changes of vien grafts intima,medial and adventitia (2w) in each group.Western blotting was used to detect the expression of p38, p-p38,Results During the 0h ~ 2w, CRP in the control group (control), single-dose group (single-dose) and the double dose group (double-doses) all of those have the upward trend, only the sham group (sham) showed in slow decline after 24h . At each time point, single-dose group, double-dose group were compared with the sham group (sham), control group (con) compared with no difference (P> 0.05), and only time in the 1w and 2w, the single dose , the double dose group were compared with the sham group was statistically difference (p = 0.025 and p = 0.015). Frome HE stain, (1w) single and double dose group endometrium, the membrane hyperplasia were significantly lower, the difference was statistically significant (p <0.05 or p <0.01); single and double dose group endometrium, the membrane hyperplasia thickness was significantly higher than sham operation group, there was significant difference (p <0.01), but each time a single, double dose group had no difference (p> 0.05) Western blotting detected the product of p38 and P-p38 showed single, double dose group was significantly lower than the control group and sham operation group, there was a significant Statistics difference.Conclusion C - reactive protein (CRP) and autologous vein graft restenosis process are closely related, which may be related with the p38MAPK pathway, CRP join in the vein arterialization in the process of restenosis, and CBS3830 did not play their inhibit. In-depth research, expected to be the new target.of prevention of vein graft restenosis and occlusion after CABG...
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