Experimental Study Of Islet Transplantation On Different Sites Of Mouse

Objective: Comparing blood glucose control effect of islet transplant on different anatomic sites(lesser omentum, axillary fossa, or under kidney capsule).Methods: Lymphocytes Separation Medium (mixture of Ficoll and sodium diatrizoate, density 1.077 g/ml) was used to purify mouse islets. After purification, dithizone was used to stain islets. Insulin release assay was used to detect the activity of purified islets. Glucose meter (One Touch Ultra) was used to detect blood glucose level in diabetic mice. Purified islets were transplanted into lesser omentum, axillary fossa, or under kidney capsule . Animals were diveded into three groups according to transplant site. Histological examination (H.E staining and Immunohistochemistry) was used to detect islet histology in different parts.Results: After purification, a large number of high purity islets were obtained; the islets were stained scarlet, oval or round; the number of islets was 102±4; glucose-stimulated insulin secretion assay revealed that purified pancreatic islets showed excellent activity; the blood glucose level of animals received islets transplant in lesser omental or under kidney capsule was no significantly different (p> 0.05), but the blood glucose of animals in the axillary fossa group was significantly different from animals in the lesser omentum group and kidney capsule group (p <0.05).Conclusion: The islets transplanted in lesser omentum or under the kidney capsule regulate blood glucose level better than those transplanted in the axillary fossa. The lesser omentum guarantees much larger capacity and better blood supply than kidney capsule. The lesser omentum accords with physiological cycle more satisfactorily, and therefore, it can be used as an ideal site of islet transplantation.