Effects Of Panaxginseng On Proliferation And Differentiation Of K562 Cells

Objective:The biological characteristics of leukemic cells is cell proliferation uncontrolled, differentiation and maturation blocked, normal apoptosis process loosened, resulting in a large number of abnormal differentiated cells proliferation. Therefore, effective method for treating leukemia is to inhibit cell proliferation and induce abnormal differentiation of the cells to mature or differentiate into more mature cells. Ginseng is a commonly used herbal medicine with a wide range of therapeutic benefits. Total saponins of Panax ginseng (TSPG) is one of the main effective components of ginseng. At present the ginseng extract from the ginseng of ginsenosides more than 30s, its pharmacological effects and action mechanism is not all the same. gensingnoside Re is a kind of ginseng saponins effective components, its quality score has accounted for about 30% of the total saponins of ginseng. ginsenoside Re induced differentiation of leukemia cells has not been reported at present. chronic myeloid leukemia cell line K562 as the research model, To investigate the growth inhibition and differentiation of K562 cells induced by total saponins of panax ginseng (TSPG) or Re.Methods:1. chronic myeloid leukemia cell line K562 as the research model, To investigate the growth inhibition and differentiation of K562 cells induced by different concentrations TSPG at different times: Different concentrations of TSPG were added to K562 cells at different times. The inhibition effects of TSPG on K562 cells were observed by MTT and trypan blue staining.2. To observe the erythroid differentiation of different concentrations TSPG or Re induced K562 cells:The differentiation of K562 cells was examined using Benzidine staining and hemoglobin synthesis test.3. The apoptosis rates of K562 cells induced for 48h by 400 mg/L TSPG were analyzed by flow cytometry. K562 cells incubated with 90μmol/L gensingnoside Re respectively last 24h, 48h,72h, Changes of CD13,CD15,GPA expressions on the surface of K562 cells were determined by flow cytometry.Results:1. TSPG have the ability to inhibit the proliferation of K562 cells in the dose-and time-dependent manners. TSPG could increase hemoglobin synthesis(P<0.05)but Benzdine-positive K562 cells induced by TSPG were not increased. TSPG at 400mg/L raised apoptosis rate of K562 cells compared with control group (P<0.05).2. Re could promote differentiation of K562 cells into erythroid linage cells in vitro: The morphological feature of K562 cells tended to be more mature; Hemoglobin in K562 cells induced by Re increased significantly; CD13 expressions on the surface of K562 cells induced by Re increased in vitro.Conclusion:1. TSPG may inhibit the proliferation of K562 cells and induce their apoptosis and differentiation.2 Re could promote differentiation of K562 cells into erythroid linage cells in vitro.