The Effects Of Co-transplatation Of Neural Stem Cells And Self-assembly Of IKVAV Peptide Nanofiber Hydrogels On The Repair Of Transversal Spinal Cord Injury In Rats

Objective:To study the effects of co-transplatation of neural stem cells (NSCs) and self-assembly of IKVAV peptide nanofiber hydrogels on the repair of transversal spinal cord injury in rats. Methed:Thirty-six SD rats were randomly devided into group A,B,and C which included 12 rats after the animal models of spinal cord completely transsected had been established for one week. DMEM/F12 fluid, NSCs and composition of cells and hydrogels were transplanted into the caulal zone of the transected spinal cord in the three groups(n=12) repectively.The function repair was evaluated by Bundle branch block (BBB) core, pathology and Imunnoflurore-scence technique. Results:The BBB scores in the three groups had significant difference at differernt time(p=0.000),and the BBB scores were significantly different among the three groups (p=0.000) after having been transplanted for two weeks. A great amount of regeneration host neuropils bridging at the two sides of the injured area, lesser glial scar and minor syringomyelia were observed in group C after having been transplanted for six weeks.A large amount of Brdu/NESTIN,Brdu/NF-200,Brdu/GFAP double positive cells were present in the injured part of spinal cord in the group B and C. There were much more Brdu/NF-200 double positive cells in group C than those in group B.However,there were much more Brdu/GFAP double positive cells in group B than those in group C.Conclusion:Both transplatation of NSCs and the composition of NSCs and the hydrogels scaffold can promote the repair of spinal cord injury in rats, co-transplantation of NSCs combined with self-assembly of IKVAV peptide nanofiber hydrogels showed more effective results.