The Experimental Study Intervention Mechanisms Of Breviscapine On The Airway Remodeling In Asthmatic Rats

ObjectiveTo observe the effects of breviscapine on airway remodeling in asthmatic rats and explore the possible mechanisms.MethodsForty SD rats were randomly divided into 4 groups(10 in each): normal group, model group, dexamethasone group, breviscapine group. The asthmatic models of rats were established by sensitized and challenged with OVA and Al(OH)3. Hematoxylin-eosin (HE) staining was used to observe the pathologic histology of lung tissue. Samples of hydroxyproline was measured with alkaline hydrolysis. Matrix metalloproteinase-9(MMP-9) and tissue inhibitor of metallo- proteinase-1(TIMP-1) in lung tissue was detected with immunohistochemical techniques. The expression of MMP-9 and TIMP-1 in lung tissue was measured with western-blot.ResultsCompared with normal group, the inflammatory cells in airway, smooth muscle were severe in model group. Statistic analysis showed that the hydroxylproline, MMP-9 and TIMP-1 in lung tissue were higher in model group than those in normal group(P<0.05). Those changes in dexamethasone group and breviscapine group were lower than those in model group(P<0.05). But its between the two groups was not significant(P>0.05).ConclusionsBreviscapine could reduce the recruitment of inflammatory cells and the formation of hydroxyproline causing collagen deposition, inhibit the expression of MMP-9 and TIMP-1 in tissue of lung, Indicating that the therapeutic mechanism of breviscapine for airway remodeling is related to reduce airway inflammation, the formation of hydroxyproline and regulate dynamic balance MMP-9?TIMP-1.