| ObjectiveTo examine the biological characteristics of human gastric cancer MGC-803 by establishing drug-resistant strains, discuss the effects of Recombinant Human Ad-p53 Injection (rAd-p53) on proliferation and apoptosis of human gastric cancer MGC-803, and explore the effects of the possible mechanism in reverse transcription.MethodsGastric cancer MGC-803 cells were induced more resistant gene expression used paclitaxel from low to the high dose; After the application of Recombinant Human Injection (rAd-p53) on gastric cancer MGC-803 cells in different concentrations (50,100,200,400,800,1600MOI) and different time (24h,48h,72h), MTT method was used to measure cell proliferation, proliferation curves were drawn; The change of cell morphology was observed by Giemsas staining; Cell cycle phase distribution and Apoptosis were determined by flow cytometry with PI; RT-PCR analysis was used to evaluate the level of MDR1 mRNA expression; Expression of MDR1-Pgp and p53 were detected by Western blot.ResultsMTT method showed that rAd-p53 inhibiting proliferation dependenced on time and dosage. The inhibiting concentration of 50% cell growth (IC50) at 24h, 48h and 72h were 1889.85MOI, 998.44MOI and 354.91MOI, respectively; Cell cycle was arrested at theG2/M phase, and apoptosis of cells could be efficiently induced by rAd-p53 at different concentrations; MDR1-Pgp protein were down-regulated and p53 protein was activated during rAd-p53 inducing in cells.ConclusionsThe biological characteristics of Resistant Cell lines were significantly different from the sensitive, multidrug resistance is mainly related with the the high expression of MDR1-Pgp;RAd-p53 significantly inhibited the proliferationof paclitaxel-resistant gastric cancer cells and induced apoptosis with dose-time dependent relationship;MDR1-Pgp protein was down-regulated in resistant gastric cancer cells by activating p53.
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