Study MiRNA-21 Plays A Role And Its Molecular Mechanism In Hormonein Dependent Prostate Cancer

ObjectiveTo study the relationship and the significance of E2 with AR-miRNA-21 pathway, Analyze the downstream regulation mechanisms of miRNA-21 in hormone-independence prostate cancer.MethodsRespectively, in the presence of E2 and E2 in the special medium does not contain cultured PC3 and then were transected with miRNA-21 inhibitor. Detect cell proliferation with CCK-8 and FCM, while the expression of gene and protein with RT-PCR and western blot.ResultsRT-PCR analysis showed that before transfection the expression of miRNA-21 in E2 environment cultured was significantly higher than in the medium without E2; In the presence of E2 the expression of miRNA-21 after transfection was significantly lower than untransfected cell group.Cck-8:in the presence of E2 the PC3 cell proliferation was significantly enhanced ; With or without E2, PC3 after transfection, the capacity of amplification was significantly weaker than the untransfected group.FCM: make sure that the miRNA-21 inhibitor is useful to mitotic cycle in PC3.AR is not different between before transfection and behind transfection (p>0.05), EGF can increase expression of AR by RT-PCR,but E2 can promote increased expression of AR.Western blot show that the expression of PTEN behind transfection is higher than before,but p-Act and MMP-2 behind transfection is lower than before.Conclusions1. E2 through inducting of AR expression in prostate cancer progression plays an important role.2. E2 may induce the expression of miRNA-21 by AR or other means.3. miRNA-21 plays an important role in the process of transfection in hormone-independence prostate cancer. Transfected with miRNA-21 inhibitor of prostate cancer cells significantly reduced the amplification capacity and prompted miRNA-21-inhibitor with prostate cancer will be a potential way of biological treatment.4. miRNA-21 negatively regulate the expression of PTEN also increased p-Akt and the expression of MMP-2, suggesting that miRNA-21 pathway may be through PTEN-pAkt/MMP-2 control the progress of prostate cancer.