The Study Of Construcing Double-membrane For Guiding Bone Regeneration With Electro Spining

Objective:To develop a double-membrane for guiding bone regeneration whose function includes both good biological activity and a certain degree of mechanical strength, and test the microstructure and the physical and chemical properties of the bilayers through a variety of experimental techniques to explore its potential values in clinical applications.Methods:First of all, we produce a series of different concentration gradient of single-membrane of PLA and PCL each by means of electro spinning, then select the best spinning solution concentration, the experiment confirmed that the best spinning solution concentration of both the two materials are 14wt%.Second,blend the mixture of collagen and polylactic acid, and finally we attempted to link the two layers of the blended membrane of collagen and polylactic acid and the fiber membrane of PCL firmly in the method of continuous electro spinning, we adjusted the component ratio of each layer membrane repeatedly to explore the cross-linking condition. After a large number of experiments, we produced a scaffold of double-membrane of type I collagen/polylactic acid and polycaprolactone; Prepare the double-membrane material samples after dry to test the fiber morphology, microstructure and mechanical properties.The SEM sample surface sprayed gold, accelerating voltage 10KV to observe fiber morphology, fiber diameter and pore size and so on.Test the tensile mechanical properties with Mechanical material testing machine, Analyze its components with X-ray diffraction machine.Results:Scanning electron microscopy test shows that both the inner and outer of the bilayer have a good fiber morphology, the inner diameter range is 100～700 nm, an average of 385nm;The scaffold has a three-dimensional porous network structure, high porosity;large pore diameter,35-128 microns, an average of 78 microns. Tensile tests showed that the average tensile strength is 29.4MPa, the average tensile maximum load is 37.6N, the average tensile stress is 13.5MPa. X-ray diffraction shows the absorption peak of the double-membrane structure, while the single-layer structure of the blend of polylactic acid and collagen membrane has no absorption peak, the absorption peak is due to the PCL crystal, and also shows that PCL has strong crystalliniry.Conclusion:1,To produce a composite double-membrane scaffold of PCL/Col-Ⅰby means of electro spinning is characterized by convenient operation, simple process and so on.2,It is feasible to build a double-membrane guiding bone regeneration with PCL,Ⅰ-collagen and polylactic acid by electrostatic spinning technology; Add a certain percentage of the same components in both the inner and the outer layer in order to have the effect of "double-anchored", which can help the two membrane to link firmly.3,The composite double-membrane of PCL/Col-Ⅰhas good mechanical properties and suitable three-dimensional structure, uniform fiber diameter, well-connected, interlinked porosity, pore size of micron. It is expected to become a good comprehensive function of new tissue engineering scaffold.4,Innovation:At the same time of constructing a new material in vitro whose microstructure and chemical composition is very similar to the natural ECM, it is also the first time to produce a double-membrane, which improves the mechanical strength, optimizes the performance of the scaffold material, breaks the limitations in clinical application because of the lack of mechanical properties of the previous scaffold.