Study Of Radioprotective Effects Of Aqueous Extract Of Sauseurea Obyallata On Immune System Of Mice

ObjectiveTo investigate the radioprotective effects of Aqueous Extract of Sauseurea Obyallata (ESO) on immune system of mice from radiation injury.MethodsImmune function tests:Four subexperiments including swimming test with weighting spleen, thymus and computing their indexs, hemolysin test, PHA-induced lymphocyte transformation test and assay for phagocytosis of peritoneal macrophage were used in the study. Mice with each subexperiment divided into 5 groups:Control group, Irradiation group (IR), Irradiation+high dose group (IR+HD), Irradiation+middle dose group(IR+MD), Irradiation+low dose group (IR+LD). ESO treated animals received intragastric administration of drugs for 14 days, including 7days before irradiation and 7days after irradiation. Other mice received the same amount of Normal Saline. All mice were irradiated with 6 Gy of x-ray exceptControl group.Micronucleus test:24h after irradiation, mice were sacrificed and measured micronucleus.Data were analyzed by SPSS 11.5.Results1. Irradiation have obvious influence on immune function and systemic state of mice.Body weights gowth slowed down, shorten swimming time, each immune index decreased significantly. In hemolysin test, two mice were dead in accident in (?)R group. The remaining mice survived.2. Swimming test showed that Control group had longer swimming time than other groups. And there was a significant difference between Control group and IR group statistically (P<0.05). ESO treated groups had longer swimming time than IR group, but there was no significant difference between them.3. Weight of immune organs showed increased splenic weight and splenic index of Control group compared with other groups, but there were no significant difference between them. The thymic weight of Control group was heavier than other groups, and that of ESO treated groups were heavier than IR group. IR+HD group have the heaviest thymus among ESO treated groups. There was a significant difference between Control group and IR group, IR+LD group, IR+MD group, respectively. And no significant difference between Control group and IR+HD group was observed. The thymus index of Control group was higher than other groups. There was a significant difference between Control group and IR group (p<0.05). Thymus indexes of ESO treated groups were higher than IR group, especially HD group had a significantly increased thymus index compared with IR group (P<0.05).4. Hemolysin test revealed that Control group had a higher OD value than other groups, and there were significant difference between them(p<0.05). There was no obvious difference between ESO treated groups and IR group.5. PHA-induced lymphocyte transformation test and assay for phagocytosis of peritoneal macrophage revealed that there were not enough Lymphocytes and peritoneal macrophages in mice which received radiation for statistical analysis. The rate of lymphocyte transformation of Control group was 28.7%.6. Micronucleus test:radiation and CTX caused a significant increase of micronucleus. ESO treated groups have lower Frequency of Micronucleus compared with IR group, especially IR+HD group/IR+MD group (p<0.01/ p<0.05).Conclusions1. Aqueous Extract of Sauseurea Obyallata may have radioprotective effects on immune system of mice by intragastric administration, and may have some effects in regulating the body state. The related performances are listed as follow:1) ESO treated groups have longer swimming time IR group.2) ESO treated groups have heavier spleen and thymus weight and higher index than IR group, especially HD group.2. The result of micronucleus test reflects that ESO has significant radio protective effects on DNA of mice, and in dose-dependent manner. Further research should be focused on compound preparing, chemical composition analysis, using dose, mode and treatment duration of the compound.